Amaxa® Cell Line Nucleofector® Kit V was purchased from Lonza (Cologne, Germany). High glucose Dulbecco’s modified eagle’s medium (DMEM) was from EuroClone S.p.A. (Milan, Italy). EPI was purchased from Selleckchem (Houston, TX, USA). Anti-β-catenin antibody was purchased from Merck KGaA, (Darmstadt, Germany). A specific antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was from NeoBioLab (Boston, MA, USA). Anti-phosphorylated Akt (Ser473, Ser472 and Ser474) antibody was purchased from R&D System (Minneapolis, MN, USA) and anti-rabbit secondary horseradish peroxidase (HRP) antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Annexin V-FITC apoptosis detection kit was from BioVision (San Jose, CA, USA). LDH-cytotoxicity Assay Kit was purchased from Abcam (Cambridge, MA, USA).
High glucose dulbecco s modified eagle s medium dmem
Manufactured by Euroclone
High glucose Dulbecco's modified eagle's medium (DMEM) is a cell culture medium formulation designed to support the growth and maintenance of a wide variety of cell types. It contains a high concentration of glucose, as well as other essential nutrients, salts, and vitamins required for cell metabolism and proliferation.
Automatically generated - may contain errors
2 protocols using high glucose dulbecco s modified eagle s medium dmem
1
Nucleofection and Biochemical Assays in Cell Lines
2
Nucleofection and Apoptosis Assay
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Amaxa® Cell Line Nucleofector® Kit V was purchased from Lonza Cologne AG (Germany). High glucose Dulbecco's modified Eagle's medium (DMEM) was from EuroClone S.p.A. (Italy). EPI was purchased from Selleckchem (USA). Anti-β-catenin antibody was purchased from Millipore (USA). A specific antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was taken from NeoBioLab (USA). Anti-phosphorylated Akt (Ser473, Ser472 and Ser474) antibody was purchased from R&D System (USA) and anti-rabbit secondary horseradish peroxidase (HRP) antibody was purchased from Santa Cruz Biotechnology (USA). Annexin V-FITC apoptosis detection kit was from BioVision (USA). LDH-cytotoxicity Assay Kit was purchased from Abcam (UK).
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