Iu1 s7134

IU1 (S7134) was obtained from Selleckchem (Houston, USA). Cycloheximide (CHX) was purchased from Sigma-Aldrich and MG132 was purchased from Abmole. Dimethylsulphoxide (DMSO) was used to dissolve these agents and stored at −20 °C.
To construct the USP14 expression plasmid, the cDNA encoding the human USP14 gene was amplified and inserted into the PcDNA3.1 plasmid with Flag tag at its N-terminus. The Luciferase reporter plasmid containing hypoxia response elements (HRE) was kindly provided by Navdeep Chandel (Addgene plasmid # 26731; http://n2t.net/addgene:26731; RRID: Addgene_26731). HA-tagged HIF1-α expression plasmid was purchased from Sion Biological Co., Ltd.

IU1 (S7134), obtained from Selleckchem (Houston, TX, USA), was dissolved into DMSO and stored at −20°C. USP14 siRNA (sc‐76817) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti‐GAPDH (MB001) was purchased from Bioworld Technology (St. Louis Park, MN, USA). Anti‐CD36 (ab133625), anti‐ABCG1 (ab52617), anti‐SR‐B1 (ab217318) and anti‐SR‐A (ab123946) were from Abcam. Anti‐Ubiquitin and anti‐USP14 were obtained from Cell Signaling Technology (MA, USA). Anti‐ABCA1 was from Santa Cruz, and Anti‐Lox‐1 was from R&D system. Oil Red O was purchased from Sigma‐Aldrich. Human oxidized low‐density lipoprotein (oxLDL) (YB‐002) and human Dil‐labelled oxidized low‐density lipoprotein (Dil‐oxLDL) (YB‐0010) were purchased from Yiyuan Biotechnologies (Guangzhou, China). Dynabeads antibody coupling kit was obtained from Life technologies. PMA (P1585) was from Sigma‐Aldrich. Anti‐CD36 (FITC) (ab39022) was purchased from Abcam.