L ascorbic acid 2 phosphate

Manufactured by Cayman Chemical

Sourced in Japan

L-Ascorbic Acid 2-phosphate is a water-soluble chemical compound. It functions as a source of ascorbic acid (vitamin C).

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Lab products found in correlation

Dexamethasone (dex), by Fujifilm (3 mentions) Recombinant human insulin, by Fujifilm (2 mentions) Forskolin, by Fujifilm (2 mentions) Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Dexamethasone (dex), by Cayman Chemical (1 mentions) α mem, by PAN Biotech (1 mentions) Bone morphogenetic protein 4 truncated bmp 4, by Fujifilm (1 mentions) Recombinant human oncostatin m osm protein, by R&D Systems (1 mentions) Ro4929097, by ChemScene (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Tgf β3, by R&D Systems (1 mentions) Bmp 2, by R&D Systems (1 mentions) L proline, by MP Biomedicals (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions)

Spelling variants of this product

L-ascorbic acid (5 citations) Ascorbic acid-2 phosphate (2 citations)

5 protocols using l ascorbic acid 2 phosphate

Directed Differentiation of hiPSC to Hepatocytes

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Subsequently, hiPSC-derived hepatocyte-like cells were further treated with 10 μM Dexamethasone (047–18863: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 10 μM Forskolin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 10 μg/mL human recombinant Insulin (093–06471: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 200 μg/mL L-Ascorbic Acid 2-phosphate (magnesium salt) (16457: Cayman Chemical, Arbor, MI), and 2 μM RO4929097 (CS-0480: ChemScene LLC, Monmouth Junction, NJ) or 10 μM DAPT (043–33581: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) in CDM5 base medium (CDM4 basal medium, supplemented with 10% KSR (10828028: ThermoFisher Scientific, Tokyo, Japan) and without the additional amino acid-rich mixture) for 6 days (day 13 to 18).
In Figure S31, differentiation into hepatocytes was performed according to the differentiation induction protocol described by Nakamura et al.

Nakashima Y., Miyagi-Shiohira C., Saitoh I., Watanabe M., Matsushita M., Tsukahara M, & Noguchi H. (2022). Induced hepatic stem cells are suitable for human hepatocyte production. iScience, 25(10), 105052.

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Osteogenic Differentiation of hPDLCs by CeO2 NPs

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Osteogenic differentiation testing was employed for the 5 g CeO₂ NPs. The sample was sterilized for 30 min under UV light at two different concentrations (C₁ = 0.125 mg and C₂ = 0.5 mg) and then seeded with the hPDLCs. Cells with number of 4 × 10⁴ were seeded onto 12-well plates 24 h before the experiment. Osteogenic medium (OM) was used for the differentiation of hPDLCs, which contained complete culture medium-CCM (α-minimum essential media (α-MEM) (PAN BIOTECHGmbH, Aidenbach, Germany); 10% FBS (BIOWEST, Nuaillé, France); and antibiotics) enhanced with 0.01 µM dexamethasone (Cayman Chemical Company, Ann Arbor, MI, USA); 50 µg/mL L-ascorbic acid 2-phosphate (Cayman Chemical Company, MI, USA); and 10 mM sodium β–glycerophosphate (Cayman Chemical Company, MI, USA) [34 (link)]. The experiment was performed with the following groups: (1) cells seeded with NPs in OM, (2) cells seeded with NPs in conventional medium (3) as a positive control, cells seeded without NPs in OM, and (4) cells without NPs in CCM as a negative control. The experiment was executed at two time points (14 and 21 days), with the OM and CCM being changed every 2 days. The impact of CeO₂ NPs on the osteogenic differentiation of hPDLCs was evaluated through alkaline phosphatase (ALP) activity and alizarin red staining (ARS).

Chatzimentor I., Tsamesidis I., Ioannou M.E., Pouroutzidou G.K., Beketova A., Giourieva V., Papi R, & Kontonasaki E. (2023). Study of Biological Behavior and Antimicrobial Properties of Cerium Oxide Nanoparticles. Pharmaceutics, 15(10), 2509.

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Liver Bud Progenitor Differentiation

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To further differentiate PFG to liver bud progenitors, one of two related types of differentiation conditions were used on days 9–12 of differentiation using 10 ng/mL Bone Morphogenetic Protein 4 (truncated) (BMP-4) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 10 ng/mL Recombinant Human Oncostatin M (OSM) Protein (295-OM-010: R&D Systems, Minneapolis, MN), 10 μM Dexamethasone (047–18863: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 2 μM RO4929097 (CS-0480: ChemScene LLC, Monmouth Junction, NJ) or 10 μM DAPT (043–33581: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 10 μM Forskolin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 10 μg/mL human recombinant Insulin (093–06471: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 200 μg/mL L-Ascorbic Acid 2-phosphate (magnesium salt) (16457: Cayman Chemical, Arbor, MI) in CDM4 medium.

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Chondrogenic Differentiation of Synovial MSCs

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In a 15 mL polypropylene tube, 2.5 × 10⁵ synovial MSCs were suspended in 1 mL of chondrogenic media consisting of DMEM high glucose (Thermo Fisher Scientific), including 10 ng/mL TGF-β3 (R&D Systems Inc.), 3.92 μg/mL dexamethasone (FUJIFILM Wako Pure Chemical Corp., Osaka, Japan), 50 μg/mL L-ascorbic acid 2-phosphate (Cayman Chemical Company, Ann Arbor, MI, USA), 40 μg/mL L-proline (MP Biomedicals, Irvine, CA, USA), 1 μg/mL sodium pyruvate (Thermo Fisher Scientific), 1% ITS-X supplement (× 100) (FUJIFILM Wako Pure Chemical Corp.), and 0.5 μg/mL BMP-2 (R&D Systems Inc.). The cells were pelleted by centrifugation at 450 g for 10 min. The pellets were cultured for 3 weeks, with the media changed every 3–4 days^{12 (link),19 (link),29 (link)}. For histological analysis, the pellets were embedded in paraffin, cut into 5 μm thick sections, stained with safranin-O and toluidine blue, and immunostained for type II collagen using the purified Anti-hCL(II) IgG antibody (KYOWA PHARMA CHEMICAL CO., LTD., Toyama, Japan)^{15 (link),38 (link)}.

Kitahashi T., Kogawa R., Nakamura K, & Sekiya I. (2022). Integrin β1, PDGFRβ, and type II collagen are essential for meniscus regeneration by synovial mesenchymal stem cells in rats. Scientific Reports, 12, 14148.

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Osteogenic Induction of hBMSCs by Exosomes

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The hBMSCs were incubated in DMEM containing 20% FBS, 2 mM b‐glycerophosphate (G‐6251, Sigma‐Aldrich), 10 nM dexamethasone (D4902, Sigma‐Aldrich), 100 mM L‐ascorbic acid 2‐phosphate (16457, Cayman), 2 mM L‐glutamine (G3126, Sigma‐Aldrich), 55 mM 2‐mercaptoethanol (M3148, Sigma‐Aldrich), and 100 mg/ml streptomycin/100 U/ml penicillin for osteogenic induction. Following incubation, the hBMSCs were treated with 50 μg/ml exosomes. Alkaline phosphatase (ALP) staining and alizarin red (ARS) staining were performed for osteogenesis analysis.

Mo C., Huang B., Zhuang J., Jiang S., Guo S, & Mao X. (2021). LncRNA nuclear‐enriched abundant transcript 1 shuttled by prostate cancer cells‐secreted exosomes initiates osteoblastic phenotypes in the bone metastatic microenvironment via miR‐205‐5p/runt‐related transcription factor 2/splicing factor proline‐ and glutamine‐rich/polypyrimidine tract‐binding protein 2 axis. Clinical and Translational Medicine, 11(8), e493.

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