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Axio imager z2 microscope system

Manufactured by Zeiss
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Sourced in Austria

The Axio Imager Z2 Microscope System is a high-performance microscope designed for advanced imaging and analysis applications. It features a modular design, allowing for customization to meet specific research or industrial requirements. The system includes a sturdy, stable stand, along with a variety of optical components and accessories to support a wide range of imaging techniques.

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Lab products found in correlation

Tissuefax, by TissueGnostics (4 mentions) Strataquest software, by TissueGnostics (2 mentions) Alexa 488 568 conjugated donkey anti mouse rabbit secondary antibodies, by Thermo Fisher Scientific (2 mentions) Fluoro gel containing dapi, by Electron Microscopy Sciences (2 mentions) Tcf7l2 primary antibody, by Merck Group (2 mentions) Image pro plus, by Media Cybernetics (1 mentions) Prism 9, by GraphPad (1 mentions) Ab209205, by Abcam (1 mentions) Tg tsa multiplex ihc assay kits, by TissueGnostics (1 mentions) Prolong diamond antifade mounting medium, by Thermo Fisher Scientific (1 mentions) Lsm 880 confocal system, by Zeiss (1 mentions) Pano 7 plex ihc kit, by Panovue (1 mentions) Ab1791, by Abcam (1 mentions) Sc 9996, by Merck Group (1 mentions) Ecl western blotting kit, by Advansta (1 mentions)

7 protocols using axio imager z2 microscope system

1

Quantitative Analysis of Skeletal Muscle Characteristics

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The cell density, nuclear area, cell area, and antibody expression in Immunohistochemical experiment, Immunofluorescent staining and Hematoxylin-Eosin staining were acquired via images by Tissue FAXS (Tissue Gnostics GmbH, Vienna Austria) with a Zeiss Axio Imager Z2 Microscope System at ×200 magnification. The cross-sectional area of 500 selected skeletal muscle fibers in the stained sections was measured and calculated using ImageJ software (version 1.8.0, National Institutes of Health) and Image-Pro Plus (version 6.0.0, media cybernetics).
Statistical analyses were executed with GraphPad Prism 9.0 software (GraphPad Software, San Diego, CA, USA) and presented as the mean value accompanied by the plus or minus standard error of the mean. The statistical significance of the differences among the three groups was determined using one-way analysis of variance (ANOVA), followed by Tukey post hoc tests, as depicted in the bar graph. A value of P <0.05 was considered to be statistically significant.
Wang C., Zhao B., Zhai J., Wang A., Cao N., Liao T., Su R., He L., Li Y., Pei X., Jia Y, & Yue W. (2023). Clinical-grade human umbilical cord-derived mesenchymal stem cells improved skeletal muscle dysfunction in age-associated sarcopenia mice. Cell Death & Disease, 14(5), 321.
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2

Multi-Immunofluorescence Tissue Analysis

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TissueFAXS (TissueGnostics GmbH, Vienna, Austria) is adopted, with a magnification of �20 Zeiss Axio imager Z2 microscope system was used for multiple immunofluorescence analysis. The quantitative analysis of tissue section images uses StrataQuest software (version 7.0.1; TissueGnostics GmbH, Vienna, Austria) to quantify the cell density of each cell, area of each cell, and expression of each cell.
Labeled antibodies include anti-CD3 (SP7), anti-CD56 (EP2567Y), anti-NKG2A (EPR23737-127), and anti-NKG2D (ab203353) for multiple staining.
Yu L., Sun L., Liu X., Wang X., Yan H., Pu Q., Xie Y., Jiang Y., Du J, & Yang Z. (2023). The imbalance between NKG2A and NKG2D expression is involved in NK cell immunosuppression and tumor progression of patients with hepatitis B virus-related hepatocellular carcinoma. Hepatology research : the official journal of the Japan Society of Hepatology, 53(5).
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3

Multiplex Immunofluorescence Staining and Analysis

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The multispectral immunofluorescence (IF) staining method was performed as previously reported [38 (link)]. The samples were stained using the following antibodies: anti-CD8 (ZCIA055, zuochengbio), anti-CD57 (ZCIA273, zuochengbio), anti-GZMA (ab209205, Abcam), and anti-GSDMB (12885-1-AP, ThermoFisher). The samples were mounted using ProLong Diamond Antifade mounting medium containing DAPI (Invitrogen) and then sliced into 5 μm sections before being placed onto adhesive microscope slides. We performed deparaffinization, rehydration, and antigen retrieval to prepare the slides for multiplexed immunofluorescence staining. TG TSA Multiplex IHC Assay Kits (TissueGnostics Asia-Pacific Ltd.) were used along with a spectral library constructed from single-staining tissue images of each reagent to guide the staining process. The imaging procedure was performed utilizing the TissueFAXS (TissueGnostics) system, in conjunction with the Zeiss Axio Imager Z2 Microscope System, at a magnification of ×20. Quantitative analysis of the stained samples involved measuring cell density, expression levels, and area per cell using StrataQuest software (version 7.1.129, TissueGnostics GmbH, Vienna, Austria).
Zhang Y., Bai Y., Ma X.X., Song J.K., Luo Y., Fei X.Y., Ru Y., Luo Y., Jiang J.S., Zhang Z., Yang D., Xue T.T., Zhang H.P., Liu T.Y., Xiang Y.W., Kuai L., Liu Y.Q, & Li B. (2023). Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence. Cell Death & Disease, 14(8), 553.
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4

Immunofluorescence of Tcf7l2 in Cholinergic Neurons

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40 μm sections containing the mHb from ChAT-Cre::tdTom mice were washed three times for 10 min in 0.1M PBS (pH 7.4). Subsequently, sections were incubated in 10% normal donkey serum (NDS) with 0.5% Triton X-100 in PBS for 30 min at RT. The sections were then incubated with the Tcf7l2 primary antibody (#17–10109, Millipore Sigma, MA, USA) in 10% NDS, 0.5% Triton X100 in PBS overnight at 4°C. Slides were then washed 4× 10 min with 0.1 M PBS. Immunoreactivity was probed using Alexa 488/568-conjugated donkey anti-mouse/rabbit secondary antibodies (1:1000; Molecular Probes, OR, USA) for 1 h at room temperature. The secondary antibodies were diluted in PBS-T containing 2% NDS. The slides were mounted with Fluoro-Gel containing DAPI (Electron Microscopy Sciences, PA, USA). The images were collected using a Zeiss AxioImager Z2 microscope system.
Duncan A., Heyer M.P., Ishikawa M., Caligiuri S.P., Liu X.A., Chen Z., di Bonaventura M.V., Elayouby K., Ables J.L., Howe W.M., Bali P., Fillinger C., Williams M., O’Connor R., Wang Z., Lu Q., Kamenecka T.M., Ma’ayan A., O’Neill H.C., Ibanez-Tallon I., Geurts A.M, & Kenny P.J. (2019). Habenular Tcf7l2 links nicotine addiction to diabetes. Nature, 574(7778), 372-377.
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5

Immunofluorescence of Tcf7l2 in Cholinergic Neurons

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40 μm sections containing the mHb from ChAT-Cre::tdTom mice were washed three times for 10 min in 0.1M PBS (pH 7.4). Subsequently, sections were incubated in 10% normal donkey serum (NDS) with 0.5% Triton X-100 in PBS for 30 min at RT. The sections were then incubated with the Tcf7l2 primary antibody (#17–10109, Millipore Sigma, MA, USA) in 10% NDS, 0.5% Triton X100 in PBS overnight at 4°C. Slides were then washed 4× 10 min with 0.1 M PBS. Immunoreactivity was probed using Alexa 488/568-conjugated donkey anti-mouse/rabbit secondary antibodies (1:1000; Molecular Probes, OR, USA) for 1 h at room temperature. The secondary antibodies were diluted in PBS-T containing 2% NDS. The slides were mounted with Fluoro-Gel containing DAPI (Electron Microscopy Sciences, PA, USA). The images were collected using a Zeiss AxioImager Z2 microscope system.
Duncan A., Heyer M.P., Ishikawa M., Caligiuri S.P., Liu X.A., Chen Z., di Bonaventura M.V., Elayouby K., Ables J.L., Howe W.M., Bali P., Fillinger C., Williams M., O’Connor R., Wang Z., Lu Q., Kamenecka T.M., Ma’ayan A., O’Neill H.C., Ibanez-Tallon I., Geurts A.M, & Kenny P.J. (2019). Habenular Tcf7l2 links nicotine addiction to diabetes. Nature, 574(7778), 372-377.
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6

Multimodal Microscopic Imaging Protocol

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Immunofluorescence staining was performed as previously described[51] In brief, fixed cells were blocked and incubated with primary antibodies overnight at 4 °C, followed by incubation with fluorescence‐conjugated secondary antibodies, according to the manufacturer's instructions. The nuclei were stained with DAPI. Fluorescence images were captured with an LSM880 confocal system (Zeiss, Carl Zeiss MicroImaging, Jena, Germany). For multiplexed immunohistochemistry, it was performed with PANO 7‐plex IHC kit (#0004100100, Panovue, Beijing, China).[53] Tissue section image was acquired with TissueFAXS (TissueGnostics GmbH, Vienna, Austria) with a Zeiss Axio Imager Z2 Microscope System, and quantitatively analyzed by HistoQuest/TissueQuest software (version 7.0.1, Vienna, Austria).
Zhou J., Zhang B., Wang H., Wang D., Zhang M., Zhang M., Wang X., Fan S., Xu Y., Zeng Q., Jia Y., Xi J., Nan X., He L., Zhou X., Li S., Zhong W., Yue W, & Pei X. (2022). A Functional Screening Identifies a New Organic Selenium Compound Targeting Cancer Stem Cells: Role of c‐Myc Transcription Activity Inhibition in Liver Cancer. Advanced Science, 9(22), 2201166.
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7

Visualizing and Quantifying WRKY63 Expression

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The vernalization or non-vernalization treated WRKY63pro::WRKY63:GFP transgenic plants were used for the fluorescence imaging. GFP fluorescence was visualized by using the Zeiss Axio Imager.Z2 microscope system (https://www.zeiss.com/microscopy). The GFP signal was quantified in the middle section of cotyledons for analyzing GFP intensity by the Zeiss ZEN software (https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html). GFP fluorescence was observed through the filter sets 46 HE (excitation at 500 nm, beamsplitter at 515 nm, and emission at 535 nm). Images were captured using a 5× objective with tile scan mode. The vernalization and non-vernalization samples were captured in the same field together and then processed and analyzed using the Zeiss ZEN software as described above.
Western blot assays were performed as previously described (Hung et al., 2018) . Anti-GFP (Santa Cruz Biotechnologies, catalog no. SC-9996; 1:3,000 dilution), anti-FLAG (sigma, M2; 1:3,000 dilution), and anti-H3 (Abcam, ab1791; 1:3,000 dilution) antibodies were used as primary antibodies for Western blot; the resulting signals were detected by using an Advansta ECL Western blotting kit (Advansta, K-12045-D20).
Hung F.Y., Shih Y.H., Lin P.Y., Feng Y.R., Li C, & Wu K. (2022). WRKY63 transcriptional activation of COOLAIR and COLDAIR regulates vernalization-induced flowering. Plant physiology, 190(1).
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