Nta 3.3 analytical software

Small EVs were analyzed by performing NTA using a NanoSight NS300 instrument (Malvern Panalytical Limited, Malvern, UK). NTA acquisition settings were optimized, and the videos were used to perform the analysis and estimate the mean size and modal size and concentration of particles. Data were processed using NTA 3.3 analytical software (Malvern Panalytical Limited, Malvern, UK).

Size distribution of EV particles was determined by nanoparticle tracking analysis (NTA) using the NanoSight NS300 analyzer. Measurements were performed in triplicate for each EV preparation using 1 μg/μl EV protein diluted 1000-fold in PBS to obtain between 20 and 100 particles per frame. Samples were introduced at ambient temperature using an automated syringe pump set to 40, with camera level set to 13, detection threshold set to 3. Acquisition time was 60s and at least 200 tracks were completed. Data were obtained and analyzed using NTA 3.3 analytical software (Malvern Instruments).

EVs diluted in PBS were analyzed using the NanoSight NS300 analyzer (Malvern Panalytical, Malvern, United Kingdom) to determine size distribution as previously described (10 (link)). Briefly, 1 µg/µL EV protein was diluted 1000-fold in PBS. Samples were introduced using an automated syringe pump set to 40, camera level to 13, and detection threshold set to 3. Acquisition time was 60 s and at least 200 tracks were completed. Data analyzed using NTA 3.3 analytical software (Malvern Panalytical).