Cells were grown at 30°C in YPD medium to saturation overnight. Cells were then diluted prior to the start of an experiment and grown for 4 h to an OD of 0.05-0.1. For microscopy experiments, cells were plated in SDC with concanavalin A (conA; Sigma-Aldrich, St Louis, MO) for 15 min to adhere them to the bottom of the glass imaging plate. Prior to imaging, the SDC was removed and replaced with a solution of SDC with 0.2M CaCl2 (Fisher Scientific, Waltham, MA). For flow cytometry experiments, cells in SDC were diluted to OD 0.1 in a media of SDC with 0.2M CaCl2 and grown in the media for the duration of the experiment. Prior to measurement, the 0.2M CaCl2 media was removed by centrifugation with 3 washes in 1X TE (Fisher Scientific, Waltham, MA).
0.2m cacl2
Manufactured by Thermo Fisher Scientific
0.2M CaCl2 is a laboratory reagent solution. It provides a standardized source of calcium chloride at a concentration of 0.2 molar.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 0.2m cacl2
1
Cell Growth and Microscopy Preparation
2
Cell Growth and Microscopy Preparation
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Cells were grown at 30°C in YPD medium to saturation overnight. Cells were then diluted prior to the start of an experiment and grown for 4 h to an OD of 0.05-0.1. For microscopy experiments, cells were plated in SDC with concanavalin A (conA; Sigma-Aldrich, St Louis, MO) for 15 min to adhere them to the bottom of the glass imaging plate. Prior to imaging, the SDC was removed and replaced with a solution of SDC with 0.2M CaCl2 (Fisher Scientific, Waltham, MA). For flow cytometry experiments, cells in SDC were diluted to OD 0.1 in a media of SDC with 0.2M CaCl2 and grown in the media for the duration of the experiment. Prior to measurement, the 0.2M CaCl2 media was removed by centrifugation with 3 washes in 1X TE (Fisher Scientific, Waltham, MA).
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