Phospho-specific anti-eIF2α, anti-eIF2α, anti-eIF4GI, anti-4EBP1 were obtained from Cell Signaling Technology (Beverly, MA). Anti-DDX3 and anti-tubulin antibodies were purchased from Abcam, and anti-ATF4 antibody from Santa Cruz Biotech. Anti-eIF4E was obtained from BD Bioscience. Anti-HuR and anti-FMRP antibodies were previously described15 (link),53 (link).
Anti eif4gi
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-eIF4GI is a primary antibody that specifically recognizes the eIF4GI protein, a subunit of the eIF4F complex involved in the initiation of protein translation. This antibody is suitable for applications such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti 4e bp1, by Cell Signaling Technology (2 mentions)
Phospho specific anti eif2α, by Cell Signaling Technology (2 mentions)
Anti atf4 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti eif4e, by BD (1 mentions)
Anti ddx3, by Abcam (1 mentions)
Anti eif2α, by Cell Signaling Technology (1 mentions)
Anti β actin, by Abnova (1 mentions)
Anti phospho 4ebp1, by Cell Signaling Technology (1 mentions)
Anti puromycin antibody, by Merck Group (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti dyskerin, by Santa Cruz Biotechnology (1 mentions)
Anti nop10, by Cusabio (1 mentions)
Anti gar1, by Proteintech (1 mentions)
Tgx stain free fastcast acrylamide solutions, by Bio-Rad (1 mentions)
Image lab v5, by Bio-Rad (1 mentions)
Mini protean tgx stain free, by Bio-Rad (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Lamin b, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
4 protocols using anti eif4gi
1
Antibody sources for eIF2α, eIF4GI, 4EBP1
2
Western Blot Analysis of EV-A71 Proteins
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The RD or SH-SY5Y whole-cell lysates were prepared by lysing with RIPA buffer and western blot was performed as Wang et al. [20 (link)] described. Anti-EV-A71 VP1 (#PAB7631-D01P) and anti-β-actin (#BE0021-1000) were obtained from Abnova and EASYBIO, respectively. Anti-ERK1/2 (#9102), anti-phospho-ERK1/2 (#9101) and anti-eIF4GI (#2858) were purchased from Cell Signaling Technology. The target protein and β-actin were detected with anti-rabbit or mouse secondary antibody conjugated with horseradish peroxidase (#BE0103-100 and # BE0108-100, EASYBIO). Specific bands were visualized with enhanced chemiluminescent substrate (ECL) and density of visualized bands was conducted using Quantity One software. Each immunoblot assay was carried out at least three times and one of them was presented.
3
Antibody Usage in Cellular Studies
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Anti-FMRP, anti-FXR1 and anti-G3BP antibodies have been previously described [22 (link)]. Anti-eIF4GI, phospho-specific anti-eIF2α, the pan anti-eIF2α, anti-4EBP1 and anti-phospho-4EBP1 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-DDX3, -PERK, -GCN2 and -α-tubulin antibodies were purchased from Abcam. Anti-m [6 (link)] A antibody was obtained from Synaptic Systems (Germany). Anti-HRI antibodies were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA). Anti-puromycin antibody was obtained from EMD Millipore (Merck, Germany). Anti-Vinculin was provided by Dr. M-E Huot (Université Laval). Anti-Dcp1a and anti-LSM14A have been described previously [23 (link),24 (link)].
4
Western Blot Analysis of Nucleolar Proteins
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Protein solutions were diluted in Laemmli loading dye (2% SDS, 8% glycerol, 62.5 mM Tris-HCl pH 6.8, 0.005% bromophenol blue, and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™; BIORAD). Proteins were transferred to nitrocellulose membranes (Amersham Protran 0.2um nc) and probed overnight at 4°C with appropriate antibodies. Detection was performed with appropriate HRP-conjugated secondary antibodies and chemiluminescent detection was performed on a ChemiDoc XRS+ Imaging System (BIORAD) using ImageLab v5.1.1 (BIORAD). The Stain-Free system makes it possible for us to check the quality and quantity of the loaded proteins, especially for RIP and polysome protein gels. The following antibodies were used: anti-Dyskerin (Santa Cruz Biotechnology, sc-373956), anti-NHP2 (Santa Cruz Biotechnology, sc-398430), anti-NOP10 (Cusabio, CSB-PA873610LD01HU), anti-GAR1 (Proteintech, 11711-1-AP), anti-RPS14 (Santa Cruz Biotechnology, sc-68873), anti-RPL5 (Bethyl, A303-933A), anti-EIF4GI (Cell Signaling, 2858S), anti-Vimentin (Cell Signaling, 5741S), anti-GAPDH (Sigma-Aldrich, G8795), anti-b-Actin (Sigma-Aldrich, A2228), Lamin B (Santa Cruz Biotechnology, sc-6216).
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