Transient gene expression in epidermal tissues from A. graveolens petioles and A.cepa scales were examined using an Olympus BX50 fluorescence microscope (http://www.olympus.co.jp/jp/ ) equipped with UPlanApo (100×) and UPlan Fl (40×, 20×) objectives and DIC optics. Images were captured using a C4742 CCD camera (Hamamatsu Photonics; http://www.hamamatsu.com/jp/ja/index.html ). Autodeblur v 9.1 software (AutoQuant Imaging; http://www.meyerinst.com/index.htm ) was used for 2D blind deconvolution. Stably transformed BY-2 cells were examined under a confocal laser scanning microscope (Leica TCS SP5 equipped with HyD detector; http://www.leica-microsystems.com ).
C4742 ccd camera
Manufactured by Hamamatsu Photonics
The C4742 is a CCD camera manufactured by Hamamatsu Photonics. It is designed to capture high-quality images and data. The camera features a CCD sensor and provides essential functionality for various scientific and industrial applications.
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Lab products found in correlation
2 protocols using c4742 ccd camera
1
Transient Gene Expression in Plant Tissues
2
Quantifying Cellular Protein Distribution
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MetaMorph 7.0.5 (Molecular Devices, Sunnyvale, CA) was used to quantify the IF signal intensity in images acquired using the UplanSApo 60×/1.35 oil-immersion lens and the Hamamatsu C4742 CCD camera. IF staining was performed as described above. Before quantification, the image-specific average out of cell fluorescence was subtracted from all images. For quantification of the cellular distribution of 53BP1, DNA images were thresholded for light objects to enable automatic creation of nuclear regions that were then transferred to 53BP1 images. After cytoplasmic 53BP1 regions for each cell were manually selected, region measurement data for each cell was logged into Excel, and the ratio of the average cytoplasmic and nuclear signal was calculated. Similarly, for Figure 5C , the DNA signal was used to create nuclear regions in the 53BP1 and γH2AX images, and Excel was used to calculate the ratios of average 53BP1 and γH2AX signal for each nucleus.
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