He (purity > 99.999%) and N2 (purity > 99.999%) were supplied by Shenyang Qianzhen Chemical Gas (Shenyang, China). Hexane (HPLC grade) and anhydrous sodium sulfate (analytical grade) were purchased from Shandong Yuwang Chemical Group (Shandong, China). Plant Genomic DNA Extraction Kits, 2X Taq master premix, and GoldView dye were purchased from Kangweishiji Bio. (Beijing, China). Agarose G-10 powder was purchased from Biowest (Hongkong, China). Synthesis of primers ITS1 and ITS4 as well as Sanger sequencing were carried out at Genewiz Bio. Ltd. (Suzhou, China). DMSO and 1% penicillin-streptomycin antibiotics were purchased from Sigma (biology grade, Burlington, VT, USA). RPMI 1640, Eagle’s Minimum Essential Medium, and 10% fetal bovine serum were purchased from Gibco (Waltham, MA, USA). Nitric Oxide Assay Kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). ELISA kits were purchased from Shanghai Joyee Biotechnics Co., Ltd. (Shanghai, China). Minocycline was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). 5-Fluorouracil was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).
Agarose g 10 powder
Manufactured by Biowest
Sourced in China
Agarose G-10 powder is a purified polysaccharide derived from seaweed. It is commonly used as a separation medium in gel electrophoresis applications to analyze and purify biomolecules such as proteins, nucleic acids, and other macromolecules.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Eagle s minimum essential medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Nitric oxide assay kit, by Beyotime (1 mentions)
Minocycline, by Meilun (1 mentions)
Sybr green 1 dye, by Thermo Fisher Scientific (1 mentions)
Fusion solo s, by Vilber (1 mentions)
3 protocols using agarose g 10 powder
1
Comprehensive Protocol for DNA Extraction and Analysis
2
Colorimetric and Gel-based RT-LAMP Assay
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RT-LAMP assay results were visually evaluated based on the color change of the solution of the reaction. The color of the negative reaction remained violet, while the positive reaction turned sky blue. In addition, agarose gel electrophoresis was also used to confirm the RT-LAMP amplification products. Agarose gel (2%, W/V) was prepared with agarose G-10 powder (Biowest) using 1 × TAE buffer (20 mM Acetic acid, 40 mM Tris, 1 mM EDTA) stained with the SYBR®Green I dye (Invitrogen™, UK). The RT-LAMP products (3 μL) were run on these gels for 40 min at 120V and 400 mA. Gels were then imaged using a Fusion SoloS. An EDGE imaging system (Vilber Lourmat, France).
3
Plant DNA Extraction and Amplification
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Helium (purity > 99.999%) and Nitrogen gas (purity > 99.999%) were supplied by Shenyang Qianzhen Chemical Gas (Shenyang, China). Hexane (HPLC grade) and anhydrous sodium sulfate (analytical grade) were purchased from Shandong Yuwang Chemical Group (Dezhou, Shandong, China). Plant Genomic DNA Extraction Kits, 2× Taq master premix, and GoldView dye were purchased from Kangweishiji Bio. Beijing, China. Agarose G-10 powder was purchased from Biowest (Hongkong, China). Primers ITS1, ITS2-F and ITS4 was synthesized by Genewiz Bio. Ltd., Suzhou, China.
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