Anti srebp1c antibody

Anti-ZBTB7A antibody (#20565s) was purchased from Cell Signaling Technology; anti-GAPDH antibody (Cat. 60004-1), mouse TNFα ELISA kit, and mouse IL-6 ELISA kits were purchased from Proteintech Inc.; anti-SREBP1c antibody was obtained from Santa Cruz Biotechnology. Oleic acid (CAS. 112-80-1, Lot. O1383) reagent was obtained from Sigma-Aldrich. TRIzol RNA extraction reagent (Cat. 10606ES60), 1st strand cDNA synthesis kit with gDNA digester (Cat. 11123ES10), qPCR SYBR Green Master Mix with high ROX (Cat. 11203ES03), immunohistochemistry kit for rabbit primary antibody (Cat. 36312ES75), and hematoxylin and eosin staining kit (Cat. 60524ES60) were purchased from Yeasen Biotech Co., Ltd; Oil Red staining kit (Cas. E607319) and the primer were obtained from Sangon Biotech (Shanghai) Co., Ltd. TG (Cas. A110-1-1), T-CHO (Cas. A111-2-1), and MDA (Cas. A003-1-1) were purchased from Nanjing Jiancheng Bioengineering Institute.

Insulin, dexamethasone, 3-isobutyl-1-methylxanthine (IBMX), sodium pyruvate, N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES), Oil Red O, and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) were purchased from Sigma-Aldrich Chemical Co., Inc. (St. Louis, MO, USA). Dubecco’s modified Eagle medium (DMEM), bovine serum (BS), fetal bovine serum (FBS), sodium pyruvate solution, and penicillin-streptomycin (PS) were purchased from Gibico (Invitrogen, Carlsbad, CA, USA). The polyclonal antibodies specific for GAPDH, PPAR-γ, C/EBPα, and p-AMPKα were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-SREBP-1c antibody was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The rabbit polyclonal antibody for AMPKα was purchased from Millipore (Darmstadt, Germany). All chemicals and solvents of analytical grade were obtained from commercial sources.