Normal cervical cell line HcerEpic and human cervical cancer cell lines, including CaSki, C‐33A HeLa, HeLa‐229, ME‐180, MS751 and SiHa, were obtained from National Collection of Authenticated Cell Cultures of China. All cell lines were maintained in DMEM (E600003; Sangon Biotech) containing 10% foetal bovine serum (E510008; Sangon Biotech) in a 5% CO2 atmosphere at 37°C.
E510008
Manufactured by Sangon
The E510008 is a laboratory equipment product from Sangon. It is designed for general laboratory use, but a detailed description of its core function is not available at this time.
Automatically generated - may contain errors
2 protocols using e510008
1
Culturing Cervical Cell Lines
2
Transwell Assay for Invasion and Metastasis
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For the purpose of monitoring invasion and metastasis, Transwell migration chambers were utilized (24-well chambers, 354572 Corning, USA). Matrigel (356234 BD, Solarbio, China) was applied to the container inserts prior to cell invasion trials. In both tests, the upper chamber of each well was seeded with 2×105 cells in 300 µL of serum-free media. A mixture of 600–800 µL DMEM (E600003, Sangon Biotech) supplemented with 10% FBS (E510008, Sangon Biotech) was used to fill the lower chambers of the Transwell. Subsequently, the cells were incubated for 24 hours. The top chambers were then rinsed with phosphate-buffered saline (PBS, E607008, Sangon Biotech), fixed with 4% paraformaldehyde (PFA, E672002, Sangon Biotech) for 30–60 minutes, and dyed with 1% crystal violet (A600331, Sangon Biotech) to obtain the desired appearance. Photomicrographs of the cell layers were taken with an Olympus BX51 microscope.
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