Anti-RxRxxS*/T*(#10001S), RSK1 (#9333S), RSK2 (#5528S), p-eIF4B (#3591S), eIF4B (#3592S) antibodies were ordered from Cell Signaling Technology. HRP anti-HA (#901519), HRP anti-Flag (#637311) antibodies were purchased from BioLegend. pRSK1 (Thr359/Ser363) (#AP0539), pRSK1 (Ser380) (#AP1147), RSK3 (#A16305), ERK1/2 (#A10613), and SUMO1 (#A19121) were purchased from ABclonal. Anti-RTA (ORF50) monoclonal mouse antibody was given by Dr. Ke Lan (Wuhan University, China) [34 (link)]. Anti-ORF65 monoclonal mouse antibody was given by Dr. Shou-Jiang Gao (University of Pittsburgh, USA) [35 (link)]. Monoclonal antibodies against ORF45 and ORF52 and polyclonal antibodies against K3, K8, and ORF55 were described previously [36 (link)–38 (link)]. EZview Red ANTI-FLAG M2 Affinity Gel (#F2426) and sodium butyrate (#B5887) were ordered from Sigma, Doxycycline (#S4163) was ordered from Selleck, Glutathione Sepharose 4B (#17-0756-01) were ordered from GE Healthcare. ClonExpress II One Step Cloning Kit (#C122-01) and HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (#R223-01) were purchased from Vazyme Biotech. Lipofectamine 3000 (#3000015) were purchased from Thermo Fisher Scientific.
Sumo1
Manufactured by ABclonal
SUMO1 is a recombinant protein produced by ABclonal. It is a Small Ubiquitin-like Modifier (SUMO) protein, which is a post-translational modification that regulates various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Sodium butyrate, by Merck Group (1 mentions)
Hiscript 2 q rt supermix for qpcr gdna wiper, by Vazyme (1 mentions)
Ezview red anti flag m2 affinity gel, by Merck Group (1 mentions)
Clonexpress 2 one step cloning kit, by Vazyme (1 mentions)
Doxycycline, by Selleck Chemicals (1 mentions)
Glutathione sepharose 4b, by GE Healthcare (1 mentions)
Erk1 2, by ABclonal (1 mentions)
Eif4b, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Hnrnpa1, by Proteintech (1 mentions)
Ecl chemiluminescence system, by Clinx (1 mentions)
2 protocols using sumo1
1
Comprehensive Antibody Validation Protocol
2
Western Blot Analysis of PBMC Lysates
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Whole-cell lysate was subjected to western blot analysis following a previously described procedure [14] . For the preparation of total cell lysates, the PBMCs were lysed in RIPA buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1.0% deoxycholate, 1% Triton X-100, 1 mM EDTA and 0.1% SDS. The samples were centrifuged (12,000×rpm, 5 min) and the supernatants were further analyzed on a 12% SDS-PAGE gel and subsequently transferred to a PVDF membrane (Millipore, Massachusetts, USA). The following antibodies were used for western blotting: Tubulin (1:1000, AC015), SUMO1 (1:500, A2130) and UBE2Q2 (1:500, A9992) and were purchased from ABclonal Biotechnology. HNRNPA1 (1:500, A12446) and TRA2B (1:500, 23832–1-AP) were purchased from Proteintech group. All the antibodies were originated from rabbit. The immunoreactive proteins were detected by ECL chemiluminescence system (Clinx, Shanghai, China) with default settings and the Tubulin was set as the normalized control.
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