Nta 3.1 analytical software

Nanoparticle Tracking Analysis (NTA) was performed to quantify EV yield and size distribution. The Nano Sight LM10 Nanoparticle Analysis System (Malvern Panalytical Ltd., Malvern, UK) and the NTA 3.1 Analytical Software (Malvern Panalytical Ltd.) were used to characterize isolated EVs. A 5 µl aliquot of stored EVs was diluted in 995 µl of 0.2 µm triple-filtered Milli-Q water (Millipore-Sigma) and injected into the NTA. A 1:200 dilution is necessary to obtain a concentration between 2 × 10⁸ and 20 × 10⁸ particle/mL, which is the suggested optimal range for NTA [25 (link),26 (link)]. Three-30 second videos were recorded and analyzed by the software. The scientific complementary metal-oxide-semiconductor (sCMOS) camera collected 739 frames at 22.0 °C. A 404 nm laser with a detection threshold of 5, determined the mean-square displacement of each nanoparticle based on its own Brownian motion. The analytical software determined EV size, mean, mode and standard deviation, as well as the number of particles per frame and milliliter of sample.

NTA experiments were carried out using NanoSight NS300 & NTA 3.1 Analytical Software (Malvern Instruments, Worcestershire, UK). LPNP-TS-DOX were diluted (5000×) in ultrapure water and placed in the NanoSight sample chamber. The suspension was irradiated for 60 s at room temperature by a laser source and light scattering. A charge-coupled device camera was used to capture images.