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Percp cy5.5 conjugated anti cd4 rm4 5

Manufactured by BD
Sourced in United States

PerCP-Cy5.5-conjugated anti-CD4+ (RM4-5) is a flow cytometry antibody reagent. It is used to detect and identify CD4+ T cells in cell samples.

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2 protocols using percp cy5.5 conjugated anti cd4 rm4 5

1

Multiparameter Immune Cell Profiling

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The following antibodies were used for cell surface and intracellular stainings: APC-conjugated anti-CD8+ (53–6.7), PerCP-Cy5.5-conjugated anti-CD4+ (RM4-5), and APC-Cy7-conjugated anti-TNFα (MP6-XT22) were purchased from BD Biosciences; PE-conjugated-anti-IL-2 (JES6-5H4), PE-CD107a-conjugated (1D4B), and PE-Cy7-conjugated anti-CD45.1 (A20) were purchased from BioLegend; FITC-conjugated-anti-IFNγ (XMG1.2) was purchased from eBioscience. A rat anti-mouse IL-6 receptor mAb (MR16-1) was kindly provided by Chugai Pharmaceutical Co.
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2

Multicolor Flow Cytometry Analysis

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Anti-CD3ε (145-2C11), anti-CD28 (37.51), alexa488-conjugated anti-IL-4 (11B11) and PerCP-Cy5.5-conjugated anti-CD4 (RM4-5) were purchased from BD Biosciences (San Diego, CA, USA). The PE-conjugated anti-IL13 (eBio13A) was from eBioscience (San Diego, CA, USA). The APC-conjugated anti-IL-17 (TC11-18H10.1) was from BioLegend (San Diego, CA, USA).
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