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Torin1

Manufactured by Beyotime
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Sourced in China

Torin1 is a small-molecule inhibitor that targets the mTOR (mammalian target of rapamycin) signaling pathway. It acts as an ATP-competitive mTOR kinase inhibitor, blocking both mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2).

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Lab products found in correlation

Anti sqstm1, by Cell Signaling Technology (1 mentions) Normal goat igg, by Santa Cruz Biotechnology (1 mentions) Anti cleaved casp3, by Cell Signaling Technology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti mtor, by Cell Signaling Technology (1 mentions) Anti flag, by Santa Cruz Biotechnology (1 mentions) Normal mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti lc3b, by Cell Signaling Technology (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions) Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions) Anti parp, by Cell Signaling Technology (1 mentions) Bafilomycin a1, by MedChemExpress (1 mentions) Mdivi 1, by MedChemExpress (1 mentions) Hy 100941, by MedChemExpress (1 mentions) Puromycin, by Merck Group (1 mentions) Chloroquine, by Selleck Chemicals (1 mentions) N acetylcysteine, by Selleck Chemicals (1 mentions) Hydroxyurea hu, by Merck Group (1 mentions) Rapamycin, by Beyotime (1 mentions) S2566, by Selleck Chemicals (1 mentions)

4 protocols using torin1

1

Investigating Autophagic Signaling Pathways

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Torin1 (SC0245) was purchased from Beyotime Biotechnology. Leupeptin (HY-18234A) and 3-MA (HY-19312) were purchased from MedChemExpress. The following antibodies were used: anti-UXT (Invitrogen, PA5-18852), anti-HA (Santa Cruz Biotechnology, sc-7392), anti-FLAG (Santa Cruz Biotechnology, sc-8036), anti-cleaved CASP3 (Cell Signaling Technology, 9664S), anti-PARP (Cell Signaling Technology, 9532S), anti-GAPDH (Santa Cruz Biotechnology, sc-32233), anti-LC3B (Cell Signaling Technology, 3868S), anti-MTOR (Cell Signaling Technology, 2972S), anti-SQSTM1 (Cell Signaling Technology, 23214S), normal mouse IgG (Santa Cruz Biotechnology, sc-2025), normal rabbit IgG (Santa Cruz Biotechnology, sc-2027), normal goat IgG (Santa Cruz Biotechnology, sc-2028), anti-p-RPS6KB1/S6K (Cell Signaling Technology, 2708S).
Pan M., Yin Y., Wang X., Wang Q., Zhang L., Hu H, & Wang C. (2020). Mice deficient in UXT exhibit retinitis pigmentosa-like features via aberrant autophagy activation. Autophagy, 17(8), 1873-1888.
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2

Morphine-Induced Autophagy Modulation

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Morphine was provided by Sun Yat-sen Memorial Hospital, which was approved by Guangdong Medical Products Administration. CCCP (10 μM, HY-100941), Mdivi-1 (20 μM, HY-15886) and Bafilomycin A1 (20 nM, HY-100558) were purchased from MedChemExpress. Torin1 (250 μM, SC0245) was purchased from Beyotime. Puromycin (Sigma, P7255) was used to select stably expressed cells.
Peng J., Pan J., Wang H., Mo J., Lan L, & Peng Y. (2022). Morphine-induced microglial immunosuppression via activation of insufficient mitophagy regulated by NLRX1. Journal of Neuroinflammation, 19, 87.
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3

Evaluating Rapamycin and Torin1 Effects

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Rapamycin and Torin1 were purchased from Beyotime Institute of Biotechnology (China). Chloroquine (CQ) and N-acetylcysteine (NAC) were purchased from Selleck Chemicals (USA). Hydroxyurea (HU) was from Sigma-Aldrich (USA). X-ray apparatus (X-RAD 225 OptiMAX) was from Precision X-ray (PXi).
Bu W., Hao X., Yang T., Wang J., Liu Q., Zhang X., Li X., Gong Y, & Shao C. (2020). Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2020, 2015920.
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4

Adipocyte Lipolysis Mechanisms

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Experiments with calf in vitro differentiated adipocytes were divided into the following 2 sections: (1) mature adipocytes were incubated with DMEM-F12 containing 10 µM ISO (S2566; Selleck Chemicals) for 3 h (Lanna and Bauman, 1999) (link) or incubated with DMEM-F12 containing 250 nM Torin1 (SC0245; Beyotime Institute of Biotechnology) for 3 h (Peterson et al., 2011) (link); and (2) to inhibit ALP, mature adipocytes were pretreated with or without 10 µg/mL leupeptin (11017101001; Sigma-Aldrich) for 4 h (Tao et al., 2019) (link) followed by treatment with ISO for additional 3 h.
Yu H., Fan M., Chen X., Jiang X., Loor J.J., Aboragah A., Zhang C., Bai H., Fang Z., Shen T., Wang Z., Song Y., Li X., Liu G., Li X, & Du X. (2022). Activated autophagy-lysosomal pathway in dairy cows with hyperketonemia is associated with lipolysis of adipose tissues. Journal of dairy science, 105(8).
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