Ezh2 5246

Manufactured by Cell Signaling Technology

Sourced in United States

EZH2 (#5246) is a rabbit monoclonal antibody that recognizes endogenous levels of total EZH2 protein. EZH2 is a histone methyltransferase that is a core component of the Polycomb Repressive Complex 2 (PRC2), which is involved in the epigenetic silencing of genes.

Automatically generated - may contain errors

Lab products found in correlation

P stat3 9145, by Cell Signaling Technology (1 mentions) Ki67 sc 23900, by Santa Cruz Biotechnology (1 mentions) Gapdh 5174, by Cell Signaling Technology (1 mentions) Docetaxel rp 56976, by MedChemExpress (1 mentions) C myc ab32072, by Abcam (1 mentions) Actin a2228, by Merck Group (1 mentions) Slug 9585, by Cell Signaling Technology (1 mentions)

3 protocols using ezh2 5246

Comprehensive Antibody Validation Protocol

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STAT3 (sc-482), AR (sc-816), GAPDH (sc-47724), tubulin (sc-23948), ChgA (sc-1488), SYP (sc-17750) and ki67 (sc-23900) antibodies were from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). H3K27me3 (#9733), H3K4me3 (#9751), EZH2 (#5246) and p-STAT3 (#9145) antibodies were from Cell Signaling Technology, Inc (Danvers, MA). The AKT (A01486), FOXA1 (EB05999), NSE (AP2780a), H3 (620–360), Methyl-K (SPC-158F), EED (A5371) and SUZ12 (AP20347b) antibodies were from OWL, Inc (San Diego, CA) and p-EZH2 antibody (IHC-00388) from Bethyl Laboratorys, Inc. (Montgomery, TX). RORα (GTX100029) antibody was from GeneTex (Irvine, CA).

Luo J., Wang K., Yeh S., Sun Y., Liang L., Xiao Y., Xu W., Niu Y., Cheng L., Maity S.N., Jiang R, & Chang C. (2019). LncRNA-p21 alters the antiandrogen enzalutamide-induced prostate cancer neuroendocrine differentiation via modulating the EZH2/STAT3 signaling. Nature Communications, 10, 2571.

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Antibody and Reagent Procurement

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Antibodies were purchased from the following companies: RRM2 (ab172476), TK1 (ab76495) and RREB1 (ab64168) were from Abcam (Cambridge, United Kingdom), AURKA (sc373856) was from Santa Cruz Biotechnology (Dallas, TX), EZH2 (#5246) and GAPDH (#5174) were from Cell Signaling Technology (Danvers, MA). Docetaxel (RP-56976) was purchased from MedChemExpress (Shanghai, China).

Dong Q., Qiu H., Piao C., Li Z, & Cui X. (2023). LncRNA SNHG4 promotes prostate cancer cell survival and resistance to enzalutamide through a let-7a/RREB1 positive feedback loop and a ceRNA network. Journal of Experimental & Clinical Cancer Research : CR, 42, 209.

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Western Blot Analysis of Protein Markers

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Proteins were extracted as previously described. 30 Protein (20 µg of total protein lysate per sample) was subjected to western blot (WB) analysis using standard protocols. EZH2 (#5246) and SLUG (#9585) antibodies were purchased from Cell Signaling Technology (USA); YY1 (ab109237), integrinβ3 (ab75872) and c-myc (ab32072) antibodies were obtained from Abcam (USA). Actin (A2228), used to normalize protein expression, was purchased from Sigma Aldrich (Italy). The secondary antibodies anti-rabbit and anti-mouse were purchased from Santa Cruz Biotechnology (USA).

Dagrada G., Rupel K., Zacchigna S., Tamborini E., Pilotti S., Cavalleri A., Fechner L.E., Laurini E., Smith D.K., Brich S, & Pricl S. (2018). Self-Assembled Nanomicelles as Curcumin Drug Delivery Vehicles: Impact on Solitary Fibrous Tumor Cell Protein Expression and Viability. Molecular pharmaceutics, 15(10).

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