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2 protocols using anti g3pdh antibody

1

Antibody-based Signaling Pathway Analysis

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Antibodies against p-AKT, AKT, cadherin, p-ERK1/2, PARP, p65, p-IκBα, IκBα, p-RSK1, RSK1, p-p27T198 or RhoA and BI-D1870 were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-α-tubulin, anti-ERK1/2, and anti-p27 antibody and cycloheximide were purchased from Sigma-Aldrich (St. Louis, MO). Anti-G3PDH antibody was purchased from GeneTex (Hsinchu, Taiwan). Anti-cSrc antibody was purchased from Abcam (Cambridge, MA). Anti-p-cSrc antibody was purchased from Cell Signaling Technology Inc. (Beverly, MA). PP2 was purchased from A. G. Scientific, Inc. (San Diego, CA). U0126 was purchased from Cayman Chemical (Ann Arbor, MI).
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2

Antibody-based Protein Expression Analysis

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Antibodies against p-AKT, AKT, cadherin, p-ERK, p21cip1, p-p38, p38, p-Raf-1, PARP, p65, p-IκBα, or IκBα were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-α-tubulin, anti-ERK, and anti-p27kip1 antibody were purchased from Sigma-Aldrich. Anti-G3PDH antibody was purchased from GeneTex (Hsinchu, Taiwan). Anti-cSrc antibody was purchased from Abcam (Cambridge, MA). Anti-p-cSrc antibody was purchased from Cell Signaling Technology Inc. (Beverly, MA). Anti-Raf-1 antibody was purchased from Epitomics (Burlingame, CA). PP2 was purchased from A. G. Scientific, Inc. (San Diego, CA). SB 203580 and Bay 11–7082 were purchased from Cayman Chemical (Ann Arbor, MI).
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