Fitc anti cd107a
FITC anti-CD107a is a flow cytometry antibody that binds to the CD107a (LAMP-1) surface marker. CD107a is a lysosome-associated membrane protein that is expressed on the cell surface during degranulation, making it a useful marker for evaluating cytotoxic cell activity.
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6 protocols using fitc anti cd107a
Intracellular Cytokine Profiling of Splenocytes
PBMC Activation and Cytokine Analysis
Intracellular Cytokine Staining of Splenocytes
Media alone and phorbol 12-myristate 13-acetate (PMA) and ionomycin stimulations (BD Bioscience) were used as negative and positive controls respectively. To test for degranulation of cells, FITC anti-CD107a (Biolegend) antibody was also added during stimulation. After 6hrs, cells were washed and stained with LIVE/DEAD violet. Surface staining was then added containing BV510 anti-CD4, APC-Cy7 anti-CD8, BV711 anti-CD62L and AF700 anti-CD44 (Biolegend). After 30 minutes incubation, cells were spun, washed, and fixed using the CytoPerm CytoWash kit following manufacturer's protocol (BD Bioscience).
Intracellular staining was then prepared using APC anti-IFNγ, BV650 anti-TNFα, PE-Cy7 anti-IL2, and PE-Cy5 anti-CD3 (Biolegend). All data was collected on a modified LSRII flow cytometer (BD Bioscience) followed by analysis with FlowJo software (BD Bioscience).
Quantifying CAR-T Cell Cytotoxicity
Activated T cell Immunophenotyping in Mice
Comprehensive PBMC Cytokine Analysis
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