Hsta00d

Blood samples were typically drawn immediately after completion of assessments. Most participants were assessed between 12:00 and 6:00 P.M, except for ten participants who were assessed between 10:00 A.M. and 12:00 P.M. Blood samples were not drawn for three participants due to time constraints in the participant’s schedule. Blood was immediately spun down at 3,000 × g for 10 minutes and plasma was frozen at −80 °C until batch-wise analyses of inflammatory markers previously associated with cancer-related fatigue in both patients and survivors (Saligan and Kim, 2012 (link); Xiao et al., 2017 ).
Plasma levels of IL-6, sIL-6r, TNF-α, sTNFRII, and IL-1ra were determined with enzyme linked immunosorbent assays (ELISAs) (R&D systems HS600B, DR600, HSTA00D, DRT200, and DRA00B, respectively). Mean minimum detectable levels were 0.039 pg/ml for IL-6, 6.5 pg/ml for sIL-6r, 0.106 pg/ml for TNF-α, 0.6 pg/ml for STNFRII, and 6.3 pg/ml for IL-1ra. One TNF-α sample that was below detection level was set at the mean minimum detectable dose. C-reactive protein (CRP) levels were determined by the MD Anderson Cancer Center core clinical laboratory using high sensitivity chemiluminescent immunometric assay run on a Siemen Immulite XPi. Standard range was 0.2 – 100 pg/mL. One sample exceeded the maximum range for CRP and was repeated at 1:10 dilution.