Tecnai 12 g2 spirit biotwin electron microscope

Manufactured by Thermo Fisher Scientific

The Tecnai-12 G2 Spirit Biotwin is an electron microscope designed for high-resolution imaging of biological specimens. It features a thermionic electron gun, a LaB6 filament, and an accelerating voltage of up to 120 kV. The microscope is equipped with a digital camera system and software for image acquisition and analysis.

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Lab products found in correlation

Osmium tetroxide, by Electron Microscopy Sciences (2 mentions) Veleta camera, by EMSIS (2 mentions) Radius software, by EMSIS (1 mentions)

Close spelling variants of this product

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2 protocols using tecnai 12 g2 spirit biotwin electron microscope

Ultrastructural Analysis of Intestinal Organoids

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Organoids of the small intestine and colon were harvested from Atf2^-/-/7^ko/ko and Atf2^wt/wt/7^wt/wt mice, immediately after 5 days of recombination and fixed in McDowell fixative containing 4% paraformaldehyde and 1% glutaraldehyde in 0.1 mol/L phosphate buffer. Organoids were postfixed with 1% osmium tetroxide (Electron Microscopy Sciences, Hatfield, PA) in cacodylate buffer. Subsequently, the samples were dehydrated in an alcohol series and embedded in epon (LX-112 resin; Ladd Research, Williston, VT). Then, 80-nm epon sections were cut and collected on formvar-coated grids, counterstained with uranyl acetate and lead citrate. Sections were examined using a Tecnai-12 G2 Spirit Biotwin electron microscope (Thermo Fisher, Eindhoven, The Netherlands), and images were taken using a Veleta camera with Radius software (EMSIS, Münster, Germany).

Meijer B.J., Giugliano F.P., Baan B., van der Meer J.H., Meisner S., van Roest M., Koelink P.J., de Boer R.J., Jones N., Breitwieser W., van der Wel N.N., Wildenberg M.E., van den Brink G.R., Heijmans J, & Muncan V. (2020). ATF2 and ATF7 Are Critical Mediators of Intestinal Epithelial Repair. Cellular and Molecular Gastroenterology and Hepatology, 10(1), 23-42.

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Ultrastructural Analysis of Epithelial Monolayers

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After cytokines stimulation and TEER measurement, transwell membranes were fixed in McDowell fixative containing 4% paraformaldehyde and 1% glutaraldehyde in 0.1 mol/L phosphate buffer for 4 h at RT and later kept at 4°C. Subsequently, samples were postfixed with 1% osmium tetroxide (Electron Microscopy Sciences, Hatfield, PA) in cacodylate buffer, dehydrated in an alcohol series, and embedded in epon (LX-112 resin; Ladd Research, Williston, VT). Subsequently, 80nm epon sections were cut and collected on formvar-coated grids, counterstained with uranyl acetate and lead citrate. Sections were analyzed using a Tecnai-12 G2 Spirit Biotwin electron microscope (Thermo Fisher, Eindhoven, The Netherlands), and images were acquired via a Veleta camera with Radius software (EMSIS, Münster, Germany).

Giugliano F.P., Navis M., Ouahoud S., Garcia T.M., Kreulen I.A., Ferrantelli E., Meisner S., Vermeulen J.L., van Roest M., Billaud J.N., Koster J., Dawood Y., de Bakker B.S., Picavet-Havik D.I., Schimmel I.M., van der Wel N.N., Koelink P.J., Wildenberg M.E., Derikx J.P., de Jonge W.J., Renes I.B., van Elburg R.M, & Muncan V. (2024). Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier. iScience, 27(6), 109909.

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