5 6 chloromethyl 2 7 dichlorodihydro fluorescein diacetate cm h2dcfda

Human prostate cancer cell lines DU-145 and PC-3 were purchased from the American Type Culture Collection (Manassas, VA, USA). Cell culture media and serum were obtained from Invitrogen Life Technologies (Carlsbad, CA, USA). p-Nitrophenyl phosphate (pNPP), arsenic trioxide, and epidermal growth factor (EGF) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Pamidronate disodium (Bedford laboratories, OH, USA) was obtained from the City of Hope Pharmacy (Duarte, CA, USA). Mouse monoclonal antibody against PTP1B, rabbit polyclonal anti-phosphotyrosine (pY99) antibody, antibody against EGFR, HRP-labeled goat anti-mouse IgG, and protein A/G plus beads were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). 5,6-Chloromethyl-2′7′-dichlorodihydrofluorescein diace-tate (CM-H₂DCF-DA) was purchased from Molecular Probes (Eugene, OR, USA). HRP-labeled anti-rabbit IgG and the enhanced chemiluminescence plus Western blotting detection system were purchased from Amersham Bioscience (Arlington Heights, IL, USA). All other reagents were obtained from Fischer Scientific (Pittsburg, PA, USA).