Immunohistochemical staining for alpha-smooth muscle actin (α-SMA) was carried out to evaluate the percentages of smooth muscle cell components (% α-SMA). Specimens were incubated overnight with primary anti-α-SMA antibody (1:100; Dako, Glostrup, Denmark) and processed with a biotin-labeled secondary antibody (1:2,000, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for counterstaining. The ratio of α-SMA staining to total area was calculated under magnification (×25) in four duplicate sections of each sample with Image Pro Plus 4.5 software (Medica Cybernetics, Silver Spring, MD, USA).
Primary anti α sma antibody
Manufactured by Agilent Technologies
Sourced in Denmark
The Primary anti-α-SMA antibody is a laboratory reagent used to detect the presence of alpha-smooth muscle actin (α-SMA) in biological samples. α-SMA is a cytoskeletal protein found in vascular smooth muscle cells and is commonly used as a marker for the identification of myofibroblasts and smooth muscle cells.
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Lab products found in correlation
2 protocols using primary anti α sma antibody
1
Quantifying Smooth Muscle Cell Presence
2
Penile Tissue Histological Analysis
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In order to calculate the smooth muscle cell (SMC)/collagen ratio, paraffin embedded slides of sectioned penile tissue were stained using Masson's trichrome as described in a previous investigation (9) (link). The SMC/collagen ratio was calculated under ×40 magnification images of the penis comprising one-half of the corpora cavernosa by analyzing the area of SMC (stained in red) and collagen (stained in blue) using ImagePro Plus 4.5 software (Medica Cybernetics, SilverSpring, MD, USA).
Immunohistochemical staining for alpha-smooth muscle actin (α-SMA) was performed to evaluate the percentages of the smooth muscle cell component (% α-SMA) as described in a previous investigation (9, (link)11) (link). Overnight incubation with primary anti-α-SMA antibody (1:100; Dako, Glostrup, Denmark) was performed. At a ×100 magnification, two fields were randomly selected on each slide, and the percentage of smooth muscle fibers in a given area was measured using Image Pro Plus 4.5software.
Immunohistochemical staining for alpha-smooth muscle actin (α-SMA) was performed to evaluate the percentages of the smooth muscle cell component (% α-SMA) as described in a previous investigation (9, (link)11) (link). Overnight incubation with primary anti-α-SMA antibody (1:100; Dako, Glostrup, Denmark) was performed. At a ×100 magnification, two fields were randomly selected on each slide, and the percentage of smooth muscle fibers in a given area was measured using Image Pro Plus 4.5software.
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