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Flr 40s w m

Manufactured by Panasonic
Sourced in Japan

The FLR 40S W/M is a fluorescent light source designed for laboratory use. It provides illumination with a 40-watt bulb and is available in both wall-mounted and mobile versions.

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2 protocols using flr 40s w m

1

Lighting Conditions for Biological Assays

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We used fluorescence tubes (FLR 40S W/M; Panasonic) for white illumination and LEDs for violet (405 nm with a full-width at half maximum [FWHM] of 14 nm), blue (470 nm [FWHM of 27 nm]), and red (660 nm [FWHM of 24 nm]) illuminations (ISL-150×150-VV, ISL-150×150-BB, and ISL-150×150-RR, respectively; CCS, Kyoto, Japan). For screening, we used a larger size panel (30 × 60 cm) of red LED (660 nm [FWHM of 18 nm]) (LED Grow Light M; Home Grown, Yokohama, Japan). For the action spectra, we used a tunable monochromatic light source (PVL-3300; Asahi Spectra, Tokyo, Japan). Fluorescence rates were measured at the surface of plates or flasks using a light meter (Model LI-250; LI-COR, NE, USA) attached to a quantum probe (LI-190; LI-COR) or a multi-channel spectrometer (MC2100; Otsuka Electronics, Osaka, Japan). Spectrograms of light sources used in this study are shown in S16 Fig. We used an undetectable level of blue LED light as a safety light for harvesting the cells in darkness.
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2

Maize Seedling Growth Regulation

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Maize (Zea mays L. cv. Cross Bantam T51) caryopses coated with thiuram to prevent fungal and microbial infection were used in this study. The plant materials were prepared as described by Soga et al. (1999) (link). Etiolated seedlings with a 25-30-mm long coleoptile and a 35-40-mm long mesocotyl were selected, and the basal 10-mm region of coleoptiles and the upper 10-mm region of mesocotyls were marked with India ink. Marked seedlings were grown either in the dark or under white fluorescent light (ca. 25 µmol m -2 s -1 at plant level; FLR40S⋅W/M; Panasonic, Japan) for 18 h at 25°C. The length and fresh weight of the marked regions were measured. All manipulations were performed under dim green light (ca. 0.09 µmol m -2 s -1 at handling level).
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