Pas stain kit

SCSP was synthesized by Wuxi MimoTopes Biotechnology (Wuxi, China) [13 (link)]. Cyclophosphamide was supplied by Hengrui Medicine (Lianyungang, China). BUN, CRE, GSH-Px, MDA, SOD, CAT determining kits and the H&E staining kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The ELISA kits and PAS stain kit were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). The primary antibodies against β-actin, NF-κB p50, NF-κB p65, IKKα, IKKβ, and IκBα were supplied by Beyotime Biotechnology (Shanghai, China). The monoclonal antibodies against Bcl-2, Bax, caspase 3, and caspase 9 were provided by Cell Signaling Technology Inc. (Beverly, MA, USA). The primary antibodies against TNF-α were supplied by Proteintech Group, Inc. (Wuhan, China). All other reagents were analytically pure.

Paraffin-embedded intestinal segments were serially sectioned into 5-μm sections. Sections were dewaxed in xylene and then rehydrated in a series of decreasing concentrations of ethanol. Sections were then stained with hematoxylin and eosin using an H&E stain kit (Solarbio, Beijing, China) according to the manufacturer’s protocol. Goblet cells were stained using a periodic acid-Schiff (PAS) stain kit (Solarbio, Beijing, China) according to the manufacturer’s instructions.

Tissues were fixed in 4% paraformaldehyde, followed by dehydration and embedding in paraffin to obtain 5 μm sections. Staining was performed using a Periodic Acid Schiff (PAS) Stain Kit (Solarbio) as per the manufacturer’s protocol. The sections were visualized and imaged using a BX53 microscope (Olympus).