Ab15603

Normal and COPD cells were cultured in a 6-well plate (45,000 ​cells/well) and treated with or without CQ or IVM for 48 ​h. The cells were trypsinized and neutralized, followed by centrifugation (500×g, 5 ​min, room temperature). The cell pellets were collected and lysed in 1X RIPA lysis buffer (ab15603, Abcam, Cambridge, UK) supplemented with EDTA-free Protease Inhibitor Cocktail (40693159001, Sigma-Aldrich). Protein concentrations were determined using a Pierce™ BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions. Cell lysate supernatants were mixed with 4X loading dye and separated by SDS-PAGE. After electrophoresis, proteins were transferred to polyvinylidene fluoride membranes. PBS containing Tween 20 and 5% (w/v) non-fat milk were applied for membranes blocking. Next, the membranes were stained with primary antibodies, after which secondary antibodies were applied to detect primary antibodies. Information on the antibodies used in western blot analyses is shown in Supplementary Table S1. Proteins were analyzed with Immobilon Western Chemiluminescent HRP Substrate (WBKLS0500; Merck Millipore, Billerica, MA, USA) using an Amersham™ Imager 600 system (GE Healthcare, Little Chalfont, UK).

The roots
of A. chinensis (DC.) Koidz. (210301)
were purchased from Guangzhou Zisun Pharmaceutical Co., Ltd. (Guangzhou,
China) and authenticated by Nengfeng Ou, the pharmacist in charge
of assessing herb quality at the Second Affiliated Hospital of Guangzhou
University of Chinese Medicine. MKN45 cells were purchased from the
China Center for Type Culture Collection (Wuhan, China). Oxaliplatin
(20220223) was purchased from Jiangsu Hengrui Pharmaceutical Co.,
Ltd. (Chengdu, China). RIPA lysis buffer (P0013B) was purchased from
Beyotime Biotechnology Co., Ltd. (Shanghai China). The monosaccharide
standards were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai,
China). Dextran standards were purchased from the American Polymers
Standards Corporation (USA). Antibodies against OCT2 (ab179808), MRP1
(ab260038), and MRP2 (ab15603) were purchased from Abcam (Cambridge,
UK). GAPDH (D16H11, CST, Danvers, MA) and Pt ICP-MS standard solution
(060078-04-01) were purchased from O2si Smart Solutions (North Charleston,
USA).