A total of four to six cartilage pellets were powdered in liquid nitrogen before protein extraction. Proteins were separated via sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) (Beyotime, China) and transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, USA). The membranes were incubated with primary antibodies including type II collagen, SRY-Box Transcription Factor 9 (SOX9), and actin (Cell Signaling Technology, USA) and then with a secondary antibody (Cell Signaling Technology). Proteins were detected using an enhanced chemiluminescence kit (Millipore).
Type 2 collagen
Manufactured by Cell Signaling Technology
Sourced in United States
Type II collagen is a structural protein that is a major component of hyaline cartilage. It provides tensile strength and support to the cartilage matrix.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence kit, by Merck Group (1 mentions)
Sds page, by Beyotime (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Primary antibodies against lc3b, by Cell Signaling Technology (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Immunofluorescence reagents, by Beyotime (1 mentions)
β galactosidase staining kit, by Beyotime (1 mentions)
Collagenase type 2, by Merck Group (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Western blotting reagents, by Beyotime (1 mentions)
2 protocols using type 2 collagen
1
Cartilage Protein Profiling by SDS-PAGE
2
Chondrocyte Autophagy and Differentiation
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Alcian dye, rat-derived recombinant PTH and type II collagenase were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Primary antibodies against LC3b, Beclin-1, P62, ATG5, type II collagen, SOX-9 and β-actin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). β-galactosidase staining kits, immunofluorescence reagents, western blotting reagents, DAPI, and cell protein extraction kits were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Finally, cell culture reagents, including Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS) and 0.25% trypsin were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA).
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