PdCl2 and CuSO4.5H2O were obtained from Sigma-Aldrich (St. Louis, MO) and Alfa Aesar (Ward Hill, MA), respectively. Radioactive 103Pd was purchased from Nordion (Ontario, Canada). PBS was purchased from Invitrogen Corporation (Carlsbad, CA). All other solvents and reagents were of analytical purity grade and were purchased from VWR (Brisbane, CA). All aqueous solutions were prepared in Millipore Milli-Q water (18 MΩ-cm) that was obtained from a Millipore Gradient Milli-Q water system (Billerica, MA).
Millipore milli q water
Manufactured by Merck Group
Sourced in Germany
Millipore Milli-Q water is a purification system that produces highly purified water. It utilizes a multi-step process to remove impurities, resulting in water with low levels of organic and inorganic contaminants.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Milli q gradient water system, by Merck Group (1 mentions)
Cuso4 5h2o, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Pdcl2, by Merck Group (1 mentions)
Rhodamine isothiocyanate, by Merck Group (1 mentions)
Isopropyl β d thiogalactoside, by Merck Group (1 mentions)
Glucose oxidase gox from aspergillus niger, by Merck Group (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
Amplex red, by Merck Group (1 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
D glucose, by Merck Group (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Horseradish peroxidase hrp, by Merck Group (1 mentions)
Melittin, by Merck Group (1 mentions)
Calcein, by Thermo Fisher Scientific (1 mentions)
Light mineral oil, by Merck Group (1 mentions)
Fluorescein, by Merck Group (1 mentions)
β casein, by Merck Group (1 mentions)
9 protocols using millipore milli q water
1
Radioisotope Synthesis and Characterization
2
Lipid and Fluorescent Probes for Membrane Studies
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1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) was obtained from Avanti Polar Lipids; Texas Red-labeled 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (TR-DHPE) and N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) were supplied by Thermal Fisher Scientific; d -(+)-glucose (≥99.5%), chloroform, melittin (≥85% HPLC, Sigma), Amplex red (≥98.0% HPLC), glucose oxidase (GOx) from Aspergillus niger (type VII, ≥100 000 units per g solid), horseradish peroxidase (HRP) (≥250 units per g solid), bovine serum albumin (BSA), rhodamine-B, rhodamine-isothiocyanate (RITC), fluorescein-isothiocyanate (FITC) and isopropyl β-d -thiogalactoside (IPTG) were purchased from Sigma Aldrich. Glass slides coated with indium tin oxide (ITO, sheet resistance ≈ 8 to 12 Ω per square, thickness ≈ 160 nm) were produced by Hangzhou Yuhong Technology. Saline solution and sucrose were supplied by Xilong Chemicals. Millipore MilliQ water with a resistivity of 18.0 MΩ cm was used throughout.
3
Lipid Membrane Formation and Analysis
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1-Palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine-5,5′-disulfonic acid (DiIC18), 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI), and calcein were purchased from ThermoFisher Scientific (Waltham, MA, USA). Chloroform used for dissolving and storing lipid stocks was purchased from Merck (Darmstadt, Germany) along with other reagents: glucose, sucrose, Triton X-100, and light mineral oil (catalog code: 330779-1L). β-Casein and bovine serum albumin (BSA) used to coat the glass coverslips were purchased from Sigma-Aldrich (Taufkirchen, Germany). Materials for producing microfluidics chips, polydimethylsiloxane (PDMS) and curing agent, were purchased as the SYLGARD® 184 silicone elastomer kit from Dow Corning (Midland, MI, USA). 1H,1H,2H,2H-Perfluorodecyltrichlorosilane was purchased from abcr GmbH (Karlsruhe, Germany). Materials for the dye leakage assay, fluorescein and α-hemolysin (α-HL), were purchased from Sigma. Millipore® MilliQ water was used to make all the aqueous solutions. Pressure was applied to the chip using a nitrogen gas line via a custom-built device. Details of the operation can be found elsewhere [34 (link)].
4
Electrochemical Measurements on Analytical-grade Compounds
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Analytical grade TBH (>99% purity) and heptahydrated Fe(II) sulfate were supplied by Sigma-Aldrich. Supporting electrolytes like sodium sulfate and lithium perchlorate were of analytical grade purchased from Fluka. High-purity Millipore Milli-Q water (resistivity > 18 MΩ cm) was used for the preparation of all solutions. Their initial pH was adjusted to 3.0 with concentrated sulfuric acid of analytical grade purchased from Merck. All the other chemicals were of HPLC or analytical grade supplied by Merck, Panreac and Sigma-Aldrich.
5
Synthesis of Aluminum Mesaconate
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All reagents used have been supplied by Sigma Aldrich (St. Louis, MO, USA), in the highest purity available, and none have undergone a subsequent purification process.
The synthesis of the aluminum mesaconate was developed following the procedure described by Adrian Gutierrez-Serpa [33 (link)]. For this purpose, a mixture of mesaconic acid (99%) (4 mmol; 520 mg) and aluminum nitrate nonahydrate (98%) (4 mmol; 1500 mg) in 60 mL of deionized water (18.2 MΩ.cm Millipore® Milli-Q® water, Merck, Darmstadt, Germany) with urea (99%) (2 mmol to 10 mmol) was made with constant stirring for 20 min. Subsequently, the solution is transferred to a 100 mL Teflon autoclave lined with a stainless-steel jacket and placed in an oven in which treatment is carried out at 150 °C for 24 h. After 24 h, the autoclave is removed from the oven and cooled to room temperature. Then the white product obtained is separated by filtration and washed repeatedly with distilled water. Finally, it is dried at 50 °C.
The synthesis of the aluminum mesaconate was developed following the procedure described by Adrian Gutierrez-Serpa [33 (link)]. For this purpose, a mixture of mesaconic acid (99%) (4 mmol; 520 mg) and aluminum nitrate nonahydrate (98%) (4 mmol; 1500 mg) in 60 mL of deionized water (18.2 MΩ.cm Millipore® Milli-Q® water, Merck, Darmstadt, Germany) with urea (99%) (2 mmol to 10 mmol) was made with constant stirring for 20 min. Subsequently, the solution is transferred to a 100 mL Teflon autoclave lined with a stainless-steel jacket and placed in an oven in which treatment is carried out at 150 °C for 24 h. After 24 h, the autoclave is removed from the oven and cooled to room temperature. Then the white product obtained is separated by filtration and washed repeatedly with distilled water. Finally, it is dried at 50 °C.
6
Radiochemical Analysis of Cesium Isotopes
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Two IAEA reference
materials, IAEA-330 (spinach) and IAEA-372 (grass), originating from
Polessko, Kyiv, Ukraine, were used for verifying the measured 135Cs/137Cs ratio in our laboratory. The “Mixed
nuclide solution 7601” from Eckert & Ziegler Nuclitec GmbH
was applied for calibrating the counting efficiency of the specific
geometry in γ-ray spectrometry for 137Cs determination.
Merck Millipore Milli-Q water (18.2 MΩ·cm) and guaranteed
grade regents, including HNO3 (69%, Carl Roth), HCl (37%,
Carl Roth), and NH3 (20%, Carl Roth), were used to prepare
solutions, which were then utilized in sample digestion and radiochemical
analysis. Ammonium molybdophosphate powder [AMP, H12Mo12N3O40P·x H2O (x ≈ 3), ACS >95%] purchased
from
Alfa Aesar was used for the Cs extraction. To remove the potentially
interfering elements from the ashed samples, Dowex 1-X8 (100–200
mesh) anion exchange resin purchased from Alfa Aesar and the cation
exchange resin AG 50 W-X8 (100–200 mesh) from BioRad Laboratories,
Inc. were prepared for sample purification.
materials, IAEA-330 (spinach) and IAEA-372 (grass), originating from
Polessko, Kyiv, Ukraine, were used for verifying the measured 135Cs/137Cs ratio in our laboratory. The “Mixed
nuclide solution 7601” from Eckert & Ziegler Nuclitec GmbH
was applied for calibrating the counting efficiency of the specific
geometry in γ-ray spectrometry for 137Cs determination.
Merck Millipore Milli-Q water (18.2 MΩ·cm) and guaranteed
grade regents, including HNO3 (69%, Carl Roth), HCl (37%,
Carl Roth), and NH3 (20%, Carl Roth), were used to prepare
solutions, which were then utilized in sample digestion and radiochemical
analysis. Ammonium molybdophosphate powder [AMP, H12Mo12N3O40P·x H2O (x ≈ 3), ACS >95%] purchased
from
Alfa Aesar was used for the Cs extraction. To remove the potentially
interfering elements from the ashed samples, Dowex 1-X8 (100–200
mesh) anion exchange resin purchased from Alfa Aesar and the cation
exchange resin AG 50 W-X8 (100–200 mesh) from BioRad Laboratories,
Inc. were prepared for sample purification.
7
Synthesis and Evaluation of Nanoparticles
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All chemicals used to synthesize NPs, phytochemical estimation, cytotoxic, antioxidant, and antibacterial studies were purchased from HiMedia (Mumbai, India). Cell culture media and fetal bovine serum were obtained from Invitrogen Life Technologies (Grand Island, NY), and pUC19 DNA was obtained from Sigma–Aldrich (St Louis, MO). Calcein AM, Ethidium Bromide, and Hoechst 33342 dyes were procured from Life Technologies (Thermo Fisher Scientific, Waltham, MA). Extracts and NPs were prepared using Millipore Milli-Q water (Merck Millipore, Massachusetts, United States). All chemicals were of analytical grade.
8
Materials and Reagents for Analytical Protocols
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If not stated otherwise, all materials were
purchased from Sigma-Aldrich. Milli-Q Millipore water with a specific
resistance of 18.2 MΩ·cm was used for all measurements
and sample preparations.
purchased from Sigma-Aldrich. Milli-Q Millipore water with a specific
resistance of 18.2 MΩ·cm was used for all measurements
and sample preparations.
9
DNA Interactions with Lipid Nanoparticles
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Unless otherwise stated, all materials were purchased from Sigma-Aldrich. The buffers were prepared with Milli-Q Millipore water with a specific resistance of 18.2 MO cm. The 2-(Nmorpholino)ethanesulfonic acid (MES) buffer was adjusted to pH 6.5 and filtered through a 0.2 mm cellulose acetate membrane before use. HBr buffer was prepared from HBr acid and adjusted to pH 3 using 1,4-diazabicyclo-(2,2,2)-octane. Lyophilised calf thymus (ct) DNA and ctDNA solution (1 mg mL À1 ) was purchased from Sigma-Aldrich. The synthesis of
amino]ethyl}-2-hexadecylpropandiamide) was described previously. 15, 19 DOPE and DPPC were purchased from Avanti Polar Lipids (Alabaster, AL, USA).
amino]ethyl}-2-hexadecylpropandiamide) was described previously. 15, 19 DOPE and DPPC were purchased from Avanti Polar Lipids (Alabaster, AL, USA).
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