Tris glycine sds page gradient 4 15 acrylamide gels

Manufactured by Bio-Rad

Tris–glycine SDS-PAGE gradient (4–15% acrylamide) gels are laboratory equipment used for protein separation and analysis through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These gels feature a gradient of acrylamide concentrations, typically ranging from 4% to 15%, which allows for the effective separation of a wide range of protein molecular weights.

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Lab products found in correlation

Nitrocellulose membrane, by Merck Group (1 mentions) P44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions) Dykddddk tag antibody flag, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

SDS-PAGE 4–15% gradient gels Tris-Glycine SDS-PAGE gels Tris-Glycine SDS gradient gels SDS-PAGE 4–15% gels 7.5% Tris-glycine acrylamide gel 10% acrylamide SDS-PAGE gel Tris-Glycine SDS-PAGE 4–20% Tris Glycine SDS gels 4–20% Tris glycine gradient gels 4–20% gradient SDS-PAGE gels

2 protocols using tris glycine sds page gradient 4 15 acrylamide gels

Cell Lysis and Western Blotting

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Cells were washed with ice-cold PBS and lysed with NP-40 lysis buffer (50 mM Tris pH 7.4, 0.5% (v/v) NP-40, 150 mM NaCl and 20 mM MgCl₂) buffer containing protease and phosphatase inhibitors. For Western blotting, 25 μg of protein was then fractionated on Tris–glycine SDS-PAGE gradient (4–15% acrylamide) gels (BioRad; #3450123), transferred onto PVDF membranes (Millipore; IPFL00010), and detected with the indicated antibodies using a LI-COR detection system.

Pinto-Fernandez A., Salio M., Partridge T., Chen J., Vere G., Greenwood H., Olie C.S., Damianou A., Scott H.C., Pegg H.J., Chiarenza A., Díaz-Saez L., Smith P., Gonzalez-Lopez C., Patel B., Anderton E., Jones N., Hammonds T.R., Huber K., Muschel R., Borrow P., Cerundolo V, & Kessler B.M. (2020). Deletion of the deISGylating enzyme USP18 enhances tumour cell antigenicity and radiosensitivity. British Journal of Cancer, 124(4), 817-830.

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Western Blot Analysis of Protein Targets

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5×10 6 HEK293T or HEK293 FRT were washed with ice-cold PBS and lysed with RIPA buffer containing protease and phosphatase inhibitors. For immunoblotting, 25 μg of protein was then fractionated on Tris-glycine SDS-PAGE gradient (4-15% acrylamide) gels (BioRad; #3450123), transferred onto nitrocellulose membranes (Millipore), and detected with the indicated antibodies using a LI-COR detection system. Primary antibody used in this study: DYKDDDDK Tag Antibody (Flag, Cell Signaling Technology, 2368), A-Raf Antibody (Cell Signaling Technology, 4432), LZTR1 (Santa Cruz Biotechnology, sc-390166), p44/42 MAPK (Erk1/2) (Cell Signaling Technology, 9107), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology, 4370) GAPDH (Sigma-Aldrich, G8795).

Damianou A., Liang Z., Lassen F., Vere G., Hester S., Charles P.D., Pinto-Fernández A., Santos-Delgado A., Fischer R., & Kessler B.M. (2021). Interplay Between KRAS and LZTR1 Protein Turnover, Controlled by CUL3/LZTR1 E3 Ubiquitin Ligase, is Disrupted by KRAS Mutations.

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