Lc ms grade acetic acid

LC/MS-grade water, acetonitrile, methanol, and 2-propanol were purchased from Kanto Chemical Co, Inc (Tokyo, Japan). LC/MS-grade ammonium acetate was purchased from Merck (Darmstadt, Germany). LC/MS-grade acetic acid was purchased from Fujifilm Wako Pure Chemical Co (Osaka, Japan). Standards were obtained from Nacalai Tesque, Inc (Kyoto, Japan), Fujifilm Wako Pure Chemical Co, and Merck. Stable isotope-labeled standards were obtained from Cayman Chemical Co (Ann Arbor, MI), Cambridge Isotope Laboratories, Inc (Tewksbury, MA), Steraloids Inc (Newport, RI), Avanti Polar Lipids Inc (Alabaster, AL), and Merck. Compound names and abbreviations of the 144 bioactive lipid standards used in this study are listed in supplemental Table S1.

Phosphate-buffered saline (PBS),
Dulbecco’s modified Eagle’s medium (DMEM) containing
glucose (25 mM), Ham’s F-12 nutrient mixture, penicillin-streptomycin
solution (10000 U/mL penicillin, 10000 μg/mL streptomycin),
10% (v/v) fetal bovine serum (FBS), and trypsin-EDTA solution (0.25%
trypsin and 1 mM EDTA) were obtained from Thermo Fisher Scientific
Inc. (Waltham, MA, U.S.A.). LC–MS-grade water, acetonitrile,
and methanol were purchased from Kanto Chemical Co., Inc. (Tokyo,
Japan). HPLC-grade chloroform and 28% (v/v) ammonium hydroxide were
purchased from Nacalai Tesque Inc. (Kyoto, Japan). LC–MS-grade
acetic acid and LC–MS-grade ammonium bicarbonate were purchased
from Fujifilm Wako Pure Chemical Co. (Osaka, Japan). LC–MS-grade
ammonium acetate was purchased from Merck (Darmstadt, Germany). Authentic
standards were obtained from Nacalai Tesque, Inc., Fujifilm Wako Pure
Chemical Co., and Merck. Stable isotope labeled authentic standards
of ¹⁸O₉-ATP (purity 92.9%), including ¹⁸O₆-ADP (6.4%) and ¹⁸O₃-AMP (0.7%),
and ¹³C₂, ¹⁵N-reduced glutathione
(GSH) (purity 95.4%), including ¹³C₄, ¹⁵N₂-oxidized glutathione (GSSG; 4.6%; Supporting Information, Figure S-1) were obtained from Taiyo
Nippon Sanso Co. Ltd. (Tokyo, Japan).

Acetic acid (LC-MS grade) and trifluoroAcetic acid (LC-MS grade) were purchased from Wako Pure Chemical (Osaka, Japan). Acetonitrile and water with 0.1% formic acid (LC-MS grade) were purchased from Thermo Fisher Scientific K.K. (Yokohama, Japan). Formic acid in water (0.1%, LC-MS grade) and formic acid in Acetonitrile (0.1%, LC-MS grade) used as mobile phases in the liquid chromatography were purchased from Sigma-Aldrich Japan (Tokyo, Japan).