Bone marrow cells were obtained from the femora, tibiae, and iliac crests of 8- to 10-week-old female B6 or B6-Ly5.1 mice. c-Kit-positive cells were enriched by anti-c-Kit antibody-conjugated MACS beads (Miltenyi Biotechnology, Germany) and stained with a lineage cocktail consisting of allophycocyanin (APC)-eFluor 780-conjugated anti-B220 (RA3–6B2, eBioscience, Waltham, MA), Gr-1 (RB6-8C5, eBioscience), and Ter-119 (TER-119, eBioscience) antibodies. Cells were also stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD34 (RAM34, eBioscience), APC-conjugated anti-c-Kit (2B8, eBioscience), phycoerythrin-cyanine 7 (PE-Cy7)-conjugated anti-Sca-1 (D7, eBioscience), brilliant violet 421-conjugated anti-CD48 (HM48-1, eBioscience), PE-conjugated anti-CD150 (TC15-12F12.2, Biolegend), and PerCP-eFluor 710-conjugated anti-CD41 (eBioMWReg30, eBioscience) antibodies. CD150+CD41−CD48−CD34−c-Kit+Sca1+Lineage− (CD150+41−34−KSL) cells, which were designated HSC1, were sorted with a FACSAria III (BD Biosciences).
Fitc conjugated anti cd34 ram34
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC)-conjugated anti-CD34 (RAM34) is a laboratory reagent used for the identification and analysis of CD34-positive cells. It consists of a fluorescein isothiocyanate (FITC) fluorescent label conjugated to the anti-CD34 monoclonal antibody clone RAM34. This product is intended for research use only.
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Lab products found in correlation
2 protocols using fitc conjugated anti cd34 ram34
1
Isolation of Murine Hematopoietic Stem Cells
2
Multiparametric Flow Cytometry Analysis of Murine Progenitor Cells
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Cells were sorted and analyzed on BD FACSAria II flow cytometers (BD Biosciences); data were analyzed using FlowJo software (TreeStar, Ashland, OR, USA). The following reagents (monoclonal antibodies unless otherwise indicated) were used for flow cytometry: fluorescein isothiocyanate (FITC)-conjugated anti-CD34 (RAM34; eBioscience, San Diego, CA, USA), FITC-conjugated anti-Ly-6A/E (Sca-1; D7, eBioscience), allophycocyanin (APC)-conjugated anti-CD45 (30F-11; eBioscience), APC-conjugated anti-CD31 (MEC13.3; BioLegend, San Diego, CA, USA), APC-conjugated anti-Ter119 (TER-119; eBioscience), phycoerythrin (PE)-conjugated anti-CD117 (c-Kit; 2B8; BD Biosciences), APC-conjugated anti-CD45.1 (A20; BioLegend), V500-conjugated anti-Ly-6A/E (Sca-1; D7, BD Biosciences), PE-Cy7-conjugated anti-CD16/32 (2.4G2; BD Biosciences), PE-CF594-conjugated CD127 (interleukin-7 receptor alpha, IL-7Rα,SB/199, BD Biosciences), brilliant violet 421-conjugated anti-CD135 (A2F10; BioLegend), 7-amino-actinomycin D necrotic cell marker (BD Biosciences), and the Mouse Lineage Antibody Cocktail (anti-CD3 , anti-CD11b, anti-B220, anti-TER 119, and anti-Gr-1 antibodies; BD Biosciences). When analyzing MSCs via flow cytometry, Mouse BD Fc Block (BD Biosciences) was used to block nonspecific binding to FcγRII/III. Each progenitor fraction was defined as follows [15] 15. Onodera, K. • Fujiwara, T. • Onishi, Y. ...
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