Approximately 25 μg of flash frozen, freshly ground ventricle cardiac tissue was used for direct quantification of intracellular dATP using the high-performance liquid chromatography (HPLC)–with tandem mass spectrometry (MS/MS) method previously described (50) . Briefly, samples were extracted 1 to 3 days before measurement using a 50% methanol solution. The supernatant was stored at -20°C until ready for injection into the HPLC-MS/MS system. A Water’s Xevo-TQ-S mass spectrometer coupled with a Water’s Acquity I-Class HPLC was used for the analysis (Milford, Massachusetts). Monitoring in negative mode via electrospray ionization was used to acquire MS/MS ions. dATP concentrations were quantified with standards and normalized to tissue weight.
Water s xevo tq s mass spectrometer
Manufactured by Waters Corporation
The Waters Xevo-TQ-S is a triple quadrupole mass spectrometer designed for high-performance quantitative and qualitative analysis. It features a high-sensitivity, high-resolution mass analyzer capable of detecting and measuring a wide range of analytes with accuracy and precision.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using water s xevo tq s mass spectrometer
1
Direct Quantification of Intracellular dATP in Cardiac Tissue
2
Quantitative Analysis of Lu AF64386
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total concentration of Lu AF64386 was determined in the plasma. Trunk blood was collected from saline and PCP pretreated rats approx. 1 week after the last experimental day 40 minutes after s.c. administration of 2.5 mg/kg Lu AF64386.
Samples were collected in K3-EDTA-coated tubes (Greiner Bio-One, Kremsmünster, Austria) and centrifuged at 3300 x G for 10 minutes at 4°C. Samples were kept at -80°C until bioanalysis. Plasma samples were precipitated with acetonitrile containing a generic internal standard (Lu AE90074). Quantitative analysis was performed by ultra-performance liquid chromatography (Acquity UPLC system; Waters, Milford, MA) followed by tandem mass spectrometry detection (Waters Xevo TQS mass spectrometer; Waters, Milford, MA) using a positive-ion electrospray ionization mode. The lower limit of concentration was 1 ng/ml.
Samples were collected in K3-EDTA-coated tubes (Greiner Bio-One, Kremsmünster, Austria) and centrifuged at 3300 x G for 10 minutes at 4°C. Samples were kept at -80°C until bioanalysis. Plasma samples were precipitated with acetonitrile containing a generic internal standard (Lu AE90074). Quantitative analysis was performed by ultra-performance liquid chromatography (Acquity UPLC system; Waters, Milford, MA) followed by tandem mass spectrometry detection (Waters Xevo TQS mass spectrometer; Waters, Milford, MA) using a positive-ion electrospray ionization mode. The lower limit of concentration was 1 ng/ml.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!