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2 protocols using imidazole ketone erastin

1

Ferroptosis Induction and Regulation

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Z-VAD-FMK, 3-methyladenine (3-MA), deferoxamine and RSL3 were obtained from MedChemExpress (NJ, USA); Z-LEHD-FMK and imidazole ketone erastin (IKE) were purchased from Selleck Chemicals (Houston, USA). Dichlorodihydro-fluorescein diacetate (DCFH-DA) was purchased from Beyotime Biotechnology (Shanghai, China). Antibodies against BCL-2, BAX, caspase 9, caspase 3, poly ADP-ribose polymerase (PARP) and β-actin were purchased from Cell Signaling Technology (Boston, MA, USA). Antibodies against SLC7A11 and GPX4 were obtained from Abcam (Cambridge, UK). Antibody against GAPDH was acquired from Goodhere Biological Technology (Hangzhou, China).
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2

Ferroptosis Induction and Inhibition Compounds

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l-Cystine, l-Methionine, and l-Glutamine were purchased from Sigma-Aldrich. Imidazole ketone Erastin (IKE), Glutathione (GSH), and Cycloheximide (CHX) were purchased from Selleck Chemical. Erastin, RSL3, and Ferrostatin-1 were purchased from Cayman Chemical. Actinomycin D and GCN2iB were from MedChemExpress. BODIPY 581/591 C11, 2-Mercaptoethanol was purchased from Thermo Fisher Scientific.
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