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4 protocols using benzyl salicylate

1

Antioxidant and Chemical Profiling

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Trolox®, α-tocopherol, butylated hydroxytoluene (BHT), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), fluorescein, sodium persulfate, sodium acetate, n-tetradecane (≥99%), quercetin-3-O-glucoside (≥90%), rosmarinic acid (≥97%), naringenin (≥95%), apigenin (≥95%), eupatilin (≥98%), ursolic acid (≥90%), rutin (≥94%), (E)-β-caryophyllene (≥98.5%), α-humulene (≥96%), (2E, 6Z)-farnesol (≥96%), benzyl benzoate (≥99%), and benzyl salicylate (≥99%) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Dipotassium phosphate was obtained from J.T. Baker (Phillipsburg, NJ, USA). Luteolin-7-O-glucoside (≥98%), luteolin-7-O-glucuronide (≥98%), cirsimaritin (≥98%), acacetin (≥98%), scopoletin (≥98%), and salvigenin (≥98%) were acquired from ChemFaces (Wuhan, China). Vitexin (≥95%) was obtained from Phytolab (Vestenbergsgreuth, Germany). Luteolin (≥98%) was purchased from Chemcruz (Santa Cruz Biotech., Dallas, TX, USA). The mixture of n-alkanes C8-C25 was obtained from AccuStandard, Inc. (New Haven, CT, USA). Helium, air, hydrogen, and nitrogen (99.995%) for GC analysis were purchased from Messer (Bucaramanga, Colombia). Ethanol (96%) was acquired from Suquin S.A.S. (Bucaramanga, Colombia). Type I water (18.2 MΩ cm) was produced using a Millipore Direct-QTM (Merck, Darmstadt, Germany) purification system.
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2

Extraction and Characterization of Natural Compounds

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MEO was purchased from Escentials of Australia (Noosaville, Queensland, Australia). 6-Pentyl-5,6-dihydropyran-2-one (known as C10 massoia lactone, ≥95%) was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Furfural, linalool, methyl eugenol, benzyl benzoate, and benzyl salicylate were acquired from Sigma-Aldrich Co. (St. Louis, MO, USA).
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3

Ecophysiological Study of Benzyl Compounds

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The work was developed at the Laboratory of Ecophysiology of Reproduction and Phytochemical Studies, Department of Botany, Center for Biological and Health Sciences-CCBS, at the Federal University of São Carlos, in the city of São Carlos-SP, Brazil. Both compounds are commercial products that were purchased from a local supplier (benzyl salicylate 98% from Sigma-Aldrich and benzyl benzoate 99% from Neon Commercial Ltda). All experiments were carried out in a climate-controlled chamber type B.O.D under controlled conditions.
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4

Comprehensive Analytical Standards Inventory

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C7-C30 saturated alkanes standard was obtained from Sigma-Aldrich (USA). The chemical standards used were p-cresol, eucalyptol, linalool (Sigma-Aldrich, USA), benzyl salicylate, methyl benzoate, trans-cinnamyl acetate, eugenol, geraniol, α-pinene, vanillin (Sigma-Aldrich, Germany), benzyl benzoate, benzyl acetate, p-cresyl methyl ether (Sigma-Aldrich, China), ethyl benzoate (Sigma-Aldrich, Mexico), trans,trans-farnesol (Sigma-Aldrich, Taiwan), α-copaene (BOC Sciences, USA) and 3-methyl-3-butenyl acetate (Symrise AG, Germany). The diluent used was nhexane (J. T. Baker, USA).
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