Female Wistar rats were bred under standard conditions at the Centro de Biología Molecular Severo Ochoa (CBMSO) in accordance with procedures approved in the Directive 2010/63/EU of the European Union with approval of the Research Ethics Committee of the Universidad Autónoma de Madrid (Comité de Ética de la Investigación UAM, CEI-UAM). Rabbit and rat antibodies against N-terminus of GlyT2 were generated in house (Zafra et al., 1995) . Other primary antibodies used were: anti-transferrin receptor (Invitrogen, #13-6800), anti-E cadherin (a gift from Amparo Cano, UAM), rabbit anti-calnexin (StressMarq Biosciences, SPC-108), mouse anti-calnexin (BD Transduction Laboratories, clone 37), anti-ubiquitin (Santa Cruz, sc-8017, clone P4D1), anti-α-tubulin (Sigma-Aldrich, clone T-6074), anti-Myc (Cell Signaling, #2276) and anti-transferrin receptor (Invitrogen). For the screening of compounds with the potential to rescue GlyT2 defective phenotypes the following chemicals were used: bupropion Neurobasal medium and B27 supplement were purchased from Invitrogen.
Spc 108
Manufactured by BD
The SPC-108 is a laboratory equipment product. It is designed to perform a specific core function within a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Anti transferrin receptor, by Thermo Fisher Scientific (2 mentions)
Mouse anti calnexin, by BD (2 mentions)
Anti myc, by Cell Signaling Technology (2 mentions)
Anti ubiquitin, by Santa Cruz Biotechnology (2 mentions)
Anti α tubulin, by Merck Group (2 mentions)
B27 supplement, by Thermo Fisher Scientific (2 mentions)
Bupropion hydrochloride, by Merck Group (1 mentions)
N arachidonoyl glycine, by Cayman Chemical (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using spc 108
1
Immunolocalization of GlyT2 in Rat Tissue
2
GlyT2 Defective Phenotypes Rescue Screening
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Female Wistar rats were bred under standard conditions at the Centro de Biología Molecular Severo Ochoa (CBMSO) in accordance with procedures approved in the Directive 2010/63/EU of the European Union with approval of the Research Ethics Committee of the Universidad Autónoma de Madrid (Comité de Ética de la Investigación UAM, CEI-UAM). Rabbit and rat antibodies against N-terminus of GlyT2 were generated in house (Zafra et al. 1995) . Other primary antibodies used were: anti-transferrin receptor (Invitrogen, #13-6800), anti-E cadherin (a gift from Amparo Cano, UAM), rabbit anti-calnexin (StressMarq Biosciences, SPC-108), mouse anti-calnexin (BD Transduction Laboratories, clone 37), anti-ubiquitin (Santa Cruz, sc-8017, clone P4D1), anti-α-tubulin (Sigma-Aldrich, clone T-6074), anti-Myc (Cell Signaling, #2276) and anti-transferrin receptor (Invitrogen). For the screening of compounds with the potential to rescue GlyT2 defective phenotypes the following chemicals were used: bupropion hydrochloride (Sigma Aldrich), ibogaine hydrochloride (LGC Standards), ALX1393 (Santa Cruz Biotechnology), N-arachidonoyl glycine (Cayman chemicals) and 4-phenylbutirate (Sigma Aldrich). All other chemicals used were from Sigma Aldrich unless otherwise noticed. Neurobasal medium and B27 supplement were purchased from Invitrogen.
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