16 0 nbd pe

DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine), and 16:0 NBD PE [1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl)
(ammonium salt)] were purchased from Avanti Polar Lipids (Alabaster,
AL). Texas Red DHPE (Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) was purchased
from Thermo Fisher Scientific UK. Vesicles were prepared as described
previously.^{25 (link)} Each lipid was dissolved
into an individual 5 mM CHCl₃ stock solution, mixed in
the desired composition, dried under nitrogen, and vacuum-desiccated
overnight. The dry film was hydrated in ultrapure water (Milli-Q)
to 1 mg/mL, vortexed for 30 min, heated in an oven at 50 °C for
30 min, and then tip-sonicated for 30 min at 4 °C to form small
unilamellar vesicles (SUVs). The SUV sample was centrifuged at 3000
rpm for 3 min to remove metal sonicator tip sediment from the SUVs.

The lipids 18: 1TAP (1, 2-dioleoyl-3-trimethylammonium-propane), DOPE (1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine), EPC, 16:0 NBD PE, and 16:0 Liss Rhod PE were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). DSPE-PEG2000-Folic acid was purchased from Nanocs (Boston, MA, USA). Chol (Cholesterol), Collagenase, and DNase I were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The dye DiI was purchased from Beyotime Biotechnology. PTX was purchased from Meilunbio^® (Dalian, China). Paclitaxel injection was purchased from HAPHARM Group Co., Ltd. (Harbin, China). The CD9, CD63, and TSG101 antibodies were purchased from Abcam (Cambridge, MA, USA).
The antibodies of flow cytometry (anti-mouse): PE-CD45, FITC-CD4, APC-CD8a, PerCP/Cyanine5.5-CD69, FITC-CD3ε, APC-CD49b (pan-NK cells), APC/Cyanine7-CD335 (NKp46), PE-CD107a (LAMP-1), APC-CD45R/B220, PE/Cyanine7-CD69, FITC-CD45, APC/Cyanine7-F4/80, PE-CD11c, PE/Cyanine7-CD86, APC anti-mouse/human CD11b, PerCP/Cyanine5.5-Ly-6G, FITC-Ly-6C, PE/Cyanine7-CD45, APC-CD25, PE-FOXP3, PE-CD86, and PerCP/Cyanine5.5-CD206 (MMR) were purchased from Biolegend (San Diego, CA, USA).