Human umbilical vein endothelial cells (huvec)

RAW264.7 cells (ATCC number: TIB-71) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) under humidified 5% CO₂ at 37°C. Human umbilical vein endothelial cells (HUVEC) (KeyGen, KG419) were maintained in EBM-2 medium (Lonza) supplemented with SingleQuot Bullet Kit (Lonza) under humidified 5% CO₂ at 37°C. For the LPS model, the cells were inoculated in a six-well plate and treated with different concentrations of PF 24 h later (10⁻⁴, 10⁻⁵, 10⁻⁶, and 10⁻⁷ M), 4 h before stimulation with LPS (Sigma, 1 μg/ml), and 12 h after stimulation with LPS, the protein of cells was extracted from the six-well plate.

Ishikawa, HUVEC and estrogen receptor-positive MCF-7 cell lines were obtained from KeyGEN Biotechnology. Cells were maintained in RPMI-1640(Gibco, Carlsbad, CA) medium containing 10% fetal bovine serum (Gibco, Carlsbad, CA) and 1% penicillin-streptomycin at 37°C in 5% CO₂, unless otherwise mentioned.

WRL-68, MCF-7, Caco-2, HUVEC, E0771, and HBL-100 were obtained from Nanjing Keygen Biotech. WRL-68, MCF-7, Caco-2, and HUVEC cells were cultured in DMEM cell culture medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, and E0771 and HBL-100 cells were cultured in the same formulation of RPMI-1640 cell culture media. All cells were maintained at 37 °C in a humidified atmosphere containing 5% CO₂ with regular sub-culturing.