Totally, 109 paired fresh HCC tissues (tumorous and adjacent normal samples) and relevant clinical information were obtained from patients undergoing hepatectomy at the First Affiliated Hospital of Nanjing Medical University (Nanjing, China) from June 2013 to July 2014. The study protocol was approved by the Institutional Ethics Committee of the First Affiliated Hospital of Nanjing Medical University, Nanjing, China. All patients had given written informed consent to participate in our study before surgery and that was conducted in accordance with the Declaration of Helsinki. All fresh tissues were collected and immediately frozen in liquid nitrogen. The diagnosis of all patients was confirmed by pathology. HepG2, Hep3B, MHCC97H, Huh7, SNU423, and SMMC-7721 human hepatoma and normal L02 cell lines were obtained from Nanjing KeyGen Biotech Co. Ltd (Nanjing, China). All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 80 U/mL of penicillin sodium at 37°C in humidified air containing 5% carbon dioxide.
Snu423
Manufactured by Keygen Biotech
Sourced in China
The SNU423 is a laboratory instrument used for the analysis and detection of specific biomolecules. It is designed to perform sensitive and precise measurements on a variety of samples.
Automatically generated - may contain errors
Lab products found in correlation
Smmc 7721, by Keygen Biotech (4 mentions)
Hep3b, by Keygen Biotech (4 mentions)
Hepg2, by Keygen Biotech (3 mentions)
Mhcc 97h, by Keygen Biotech (3 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Human normal l02 cell line, by Keygen Biotech (2 mentions)
Mhcc 97l, by Keygen Biotech (1 mentions)
4 protocols using snu423
1
Comprehensive HCC Tissue Collection and Cell Lines
2
Hepatocellular Carcinoma Tissue and Cell Collection
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110 paired HCC fresh tissues consist of tumors and adjacent normal samples were obtained from patients who underwent liver resection at the Liver Transplantation Center in The First Affiliate Hospital of Nanjing Medical University between October 2014 and November 2015. All patients provided their written informed consent to participate in this study. The fresh tissue samples which were confirmed by the histopathological examination were collected in the operating room and processed immediately. Each sample was frozen and stored at liquid nitrogen. The HepG2, SNU423, SMMC-7721, Hep3B, 97H, 97L and Huh7 HCC cell lines and the human normal L02 cell line used in this study were obtained from KeyGen (Nanjing KeyGen Biotech Co.Ltd, China). All of the cells were cultured in DMEM medium(GIBCO, Carlsbad, USA) pre-treated with 10% fetal bovine serum, 80 U/ml of penicillin sodium at 37°C in humidified air containing 5% carbon dioxide.
3
Hepatocellular Carcinoma Tissue Analysis
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Data were obtained from 108 paired HCC fresh tissues (including tumors and adjacent normal samples) and 12 normal liver tissues (hepatic hemangioma patients) acquired between August 2012 and September 2013 at The First Affiliated Hospital of Nanjing Medical University (Nanjing, Jiangsu, China). Informed consent for tissue analysis was obtained before surgery. The study was approved by the Institutional Ethics Committee of Nanjing Medical University. All research was performed in compliance with government policies and the Helsinki declaration. All experiments were undertaken with the understanding and written consent of each subject. The Huh7, SNU-423, MHCC-97H, MHCC-97L, SMMC-7721, Hep3B, HepG2 human hepatoma cell lines, and the human normal liver L02 cell line were obtained from KeyGen (Nanjing KeyGen Biotech Co., Ltd., Jiangsu, China). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) at 37 °C in humidified air containing 5% carbon dioxide.
4
HCC Tissue Collection and Cell Culture
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A total of 90 paired tissues of HCC tumours and adjacent normal samples and related clinical information were obtained from patients undergoing liver resection at the Liver Transplantation Center in The First Affiliate Hospital of Nanjing Medical University between October 2012 and March 2013. This research was approved by our Institutional Ethics Committee. All patients in our study offered their informed consent to take part in our study prior to surgery. Fresh tissues were collected and immediately frozen in liquid nitrogen. The diagnosis of all patients was histopathologically confirmed. The human hepatoma cell lines, HepG2, SMMC‐7721, SNU‐423, Hep3B, Huh7, MHCC‐97H and the human normal L02 cell line were obtained from KeyGen (Nanjing KeyGen Biotech Co., Ltd., Jiangsu, China). The cells were cultured in DMEM medium (Gibco, Carlsbad, CA, USA) supplemented with 10% foetal bovine serum (FBS) and maintained in an atmosphere of 5% CO2 at 37°C.
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