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Snu 1

Manufactured by American Type Culture Collection
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The SNU-1 is a laboratory equipment manufactured by American Type Culture Collection. It is designed to facilitate the cultivation and maintenance of various types of cell cultures. The core function of the SNU-1 is to provide a controlled and consistent environment for the growth and propagation of cell lines.

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Lab products found in correlation

Nci n87, by American Type Culture Collection (41 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (40 mentions) Kato 3, by American Type Culture Collection (39 mentions) Snu 16, by American Type Culture Collection (34 mentions) Mkn45, by American Type Culture Collection (24 mentions) Ges 1, by American Type Culture Collection (19 mentions) Snu 5, by American Type Culture Collection (19 mentions) Hgc 27, by American Type Culture Collection (16 mentions) Mkn 28, by American Type Culture Collection (14 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (10 mentions) Rpmi 1640, by Thermo Fisher Scientific (9 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (9 mentions) Sgc 7901, by American Type Culture Collection (9 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (8 mentions) Hs746t, by American Type Culture Collection (7 mentions) Snu 1, by Thermo Fisher Scientific (7 mentions) Mgc 803, by American Type Culture Collection (7 mentions) Bgc 823, by American Type Culture Collection (7 mentions) Hek293t, by American Type Culture Collection (6 mentions) Rpmi 1640 medium, by Merck Group (5 mentions)

Close spelling variants of this product

SNU-1 (CRL-5971) (2 citations) SNU-1 cells (2 citations)

105 protocols using snu 1

1

Cell Culture of Gastric Cell Lines

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The human gastric mucosal epithelial cell line (GES-1), GC cell lines (AGS, NCI-N87, MKN-45, SNU-1, and SNU-16), and HEK293T cells were purchased from ATCC and authenticated by STR profiling. These cells were cultured in F12K, RPMI 1640 or DMEM (Gibco, USA) medium with 10% FBS (Gibco) and 1% penicillin-streptomycin at 37 °C in a 5% CO2 atmosphere.
Liu J., Niu L., Hao J., Yao Y., Yan M, & Li H. (2023). circIPO7 dissociates caprin-1 from ribosomes and inhibits gastric cancer cell proliferation by suppressing EGFR and mTOR. Oncogene, 42(13), 980-993.
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2

Cell Culture for Gastric Cancer Research

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AGS, SNU1, MKN45, and 293T cell lines were purchased from ATCC (Manassas, VA, USA). MKN28 and NUGC3 cell lines were obtained from the Health Science Research Resources Bank (Tokyo, Japan). BGC823, MGC803, and SGC7901 cell lines were obtained from the Cell Research Institute (Shanghai, China). The cells were cultured in RPMI-1640 medium (GIBCO, Carlsbad, NY, USA), supplemented with 10% (v/v) fetal calf serum (GIBCO, NY, USA) and antibiotics at 37°C in a humidified 5% CO2 atmosphere.
Antibodies for Vimentin, E-cadherin, N-cadherin, Akt, phospho-Akt (Ser-473), ERK1/2, phospho-ERK1/2 (Thr-202/Tyr-204), Snail, and GAPDH were purchased from Cell Signaling Technology (Beverly, MA). Anti-MELK antibody and puromycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). OTSSP167 was purchased from Medchem Express (Beijing, China).
Li S., Li Z., Guo T., Xing X.F., Cheng X., Du H., Wen X.Z, & Ji J.F. (2015). Maternal embryonic leucine zipper kinase serves as a poor prognosis marker and therapeutic target in gastric cancer. Oncotarget, 7(5), 6266-6280.
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3

Gastric Cancer Tissue Profiling

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Stomach biopsy was performed. Tumor and adjacent normal tissues were also collected from each patient through biopsy. Human gastric cancer cell lines SNU-1 and AGS were used to perform all in vitro cell experiments. AGS and SNU-1 cells were purchased from ATCC (Manassas, VA, USA). Cells were cultivated with RPMI-1640 Medium containing 10% fetal bovine serum (FBS), and cell culture conditions were 37°C and 5% CO2.
Cai Y., Li Y., Sun B., Wang H., Zhang W., Zhao Y., Zhao H., Zhang J., Xu J, & Wang Y. (2020). LncRNA PTCSC3 and lncRNA HULC Negatively Affect Each Other to Regulate Cancer Cell Invasion and Migration in Gastric Cancer. Cancer Management and Research, 12, 8535-8543.
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4

Gastric Cancer Cell Line Cultivation

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Human gastric cancer cell lines AGS (ATCC CRL-1739™ and gastric adenocarcinoma) and SNU-1 (CRL-5971™, gastric carcinoma) were purchased from ATCC. All cell lines were cultured according to the manufacturer’s instructions. Cells were harvested during logarithmic growth phase for the following experiments.
Zhang J., Xu J., Dong Y, & Huang B. (2018). Down-regulation of HIF-1α inhibits the proliferation, migration, and invasion of gastric cancer by inhibiting PI3K/AKT pathway and VEGF expression. Bioscience Reports, 38(6), BSR20180741.
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5

Gastric Cancer Cell Line Culture

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Gastric cancer cell lines AGS (ATCC: CRL-1739), SNU-1 (ATCC: CRL-5971), HS-746T (ATCC: HTB-135), KATOIII (ATCC: HTB-103) and NCI-N87 (ATCC: CRL-5822), were obtained from American Type Culture Collection (Manassas, VA, USA). Other gastric cancer cell lines MKN-45, MKN-28, BGC-823 and SGC-7901 and immortalized human gastric epithelial cell line GES-1 were preserved in our institute. All cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 80 U/ml penicillin, and 100 mg/ml streptomycin.
Huang J., Zhang L., He C., Qu Y., Li J., Zhang J., Du T., Chen X., Yu Y., Liu B, & Zhu Z. (2014). Claudin-1 enhances tumor proliferation and metastasis by regulating cell anoikis in gastric cancer. Oncotarget, 6(3), 1652-1665.
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6

Gastric Cancer Cell Line Treatments

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Gastric cancer cell line (AGS, KATO III, MKN28, MKN45, SNU1, NCI-N87, purchased from ATCC, Manassas, VA) and an immortalized gastric epithelial cell line, GES (a kind gift from Dr. Jun Yu, The Chinese University of Hong Kong, Hong Kong) were maintained in RPMI 1640 (Gibco, Waltham, MA) supplemented with 10% FBS (Gibco) and 1% Penicillin-Streptomycin (Gibco). All cells were maintained at 37°C, with 5% CO2, under a humidified incubator. Cells were treated with STAT3 inhibitor, JSI-124 (Sigma, St. Louis, MO) for 2 days and harvested for RNA extraction.
Tran M.T., Yeh K.T., Chuang Y.M., Hsu P.Y., Low J.T., Kumari H., Lee Y.T., Chen Y.C., Huang W.H., Jin H., Lin S.H, & Chan M.W. (2021). Methylomic analysis identifies C11orf87 as a novel epigenetic biomarker for GI cancers. PLoS ONE, 16(4), e0250499.
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7

Gastric Cancer Cell Culture Protocol

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Gastric cancer cell lines: AGS (ATCC CRL-1739), KatoIII (ATCC HTB-103), MKN7 (JCRB1025), MKN45 (JCRB0254), NCI-N87 (ATCC CRL-5822), NUGC-4 (JCRB0834), SNU1 (ATCC CRL-5971), St2957 (CVCL_9557) and St3051 (CVCL_9558) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and supplemented with 10 % (v/v) FCS and 1% (v/v) Pen/Strep. Chinese hamster ovary cells (CHO) (ATCC CCL-61) and vector control CHO-CEACAM1-4L (CHO CC1) [21 (link)] were cultured in DMEM and supplemented with 10 % (v/v) FCS and 1% (v/v) Pen/Strep. Cells were kept in a humidified atmosphere at 37 °C and 5% (v/v) CO2. Regular mycoplasma testing was performed.
Taxauer K., Hamway Y., Ralser A., Dietl A., Mink K., Vieth M., Singer B.B., Gerhard M, & Mejías-Luque R. (2021). Engagement of CEACAM1 by Helicobacter pylori HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells. Microorganisms, 9(8), 1748.
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8

Establishment and Characterization of Gastric Cancer Cell Lines

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Establishment of tissue culture line SB.msgc-1 at our institution was previously reported [14 (link)]. Gastric cancer lines SNU-1, SNU-5, SNU-16, KATO III, AGS, NCI-N87, and BxPC-3 and HeLa cells were purchased from ATCC (American Type Culture Collection (ATCC), Manassas, VA). Antibodies for immunofluorescence studies included mouse anti-E-cadherin primary antibody (BD Transduction Laboratories, San Jose, CA), CEA/CD66e antibody (Cell Signaling, Danvers, MA), mouse IgG2a, κ (BD Biosciences, San Jose, CA), mouse (G3A1) mAb IgG1 isotype control (Cell Signaling, Danvers, MA), and Alexa Fluor ® 488 goat anti-mouse IgG (H + L) antibody (Life Technologies, Frederick, MD). Etoposide, mitoxantrone, and PI-103 were purchased from SelleckChem (Houston, TX).
Chen I., Mathews-Greiner L., Li D., Abisoye-Ogunniyan A., Ray S., Bian Y., Shukla V., Zhang X., Guha R., Thomas C., Gryder B., Zacharia A., Beane J.D., Ravichandran S., Ferrer M, & Rudloff U. (2017). Transcriptomic profiling and quantitative high-throughput (qHTS) drug screening of CDH1 deficient hereditary diffuse gastric cancer (HDGC) cells identify treatment leads for familial gastric cancer. Journal of Translational Medicine, 15, 92.
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9

Cell Lines for Gastrointestinal Cancer Research

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Human esophageal adenocarcinoma cell line ESO26 was obtained from Sigma-Aldrich (St. Louis, MO), human colorectal cancer (CRC) cell lines (SW480, HCT116, SW620, RKO, SK-CO-1, LS180, and LS153) and human gastric cancer cell lines (AGS, SNU-1, MKN45) were obtained from ATCC (Manassas, VA) and Riken Cell Bank (Tsukuba, Japan); gastric cancer cell line STKM2 was a generous gift from Dr. Alexander Zaika, University of Miami; gastric cancer cell line SNU-601 was purchased from the Korean Cell Line Bank (Jongno-gu, Seoul). AGS and STKM2 were maintained in F12 medium (GIBCO, Carlsbad, CA); CRC cell lines were maintained in RPMI-1640 medium (GIBCO); SNU-1, MKN45 and ESO26 were maintained in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO). All cell lines were supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). All cell lines were ascertained to conform to the original in vitro morphologic characteristics and were authenticated by Genetica DNA Laboratories using short tandem repeat profiling (Genetica DNA Laboratories).
Wang-Bishop L., Chen Z., Gomaa A., Lockhart A.C., Salaria S., Wang J., Lewis K.B., Ecsedy J., Washington K., Beauchamp R.D, & El-Rifai W. (2018). Inhibition of AURKA Reduces Proliferation and Survival of Gastrointestinal Cancer Cells With Activated KRAS by Preventing Activation of RPS6KB1. Gastroenterology, 156(3), 662-675.e7.
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10

GC Cell Line Cultivation Protocol

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AGS (RRID:CVCL_0139), KATO-III (RRID:CVCL_0371), and SNU-1 (RRID:CVCL_0099) GC cell lines were purchased from ATCC (Manassas, VA, United States) and were cultured in their specific complete medium according to manufacturer instructions. GC cells were incubated at 37°C in 5% CO2; medium was changed daily, and cells were split every 2-3 days routinely.
Laurino S., Mazzone P., Ruggieri V., Zoppoli P., Calice G., Lapenta A., Ciuffi M., Ignomirelli O., Vita G., Sgambato A., Russi S, & Falco G. (2021). Cationic Channel TRPV2 Overexpression Promotes Resistance to Cisplatin-Induced Apoptosis in Gastric Cancer Cells. Frontiers in Pharmacology, 12, 746628.
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