HT-1080 human fibrosarcoma, HT-29 human colon carcinoma, and MCF7 human breast carcinoma were purchased from the American Type Culture Collection (Manassa, VA, USA). M21 human skin melanoma cells were provided by Dr. David Cheresh (University of California, San Diego School of Medicine, CA) Cells were cultured in DMEM medium containing sodium bicarbonate, high glucose concentration, glutamine and sodium pyruvate (Hyclone, Logan, UT, USA) supplemented with either 5 or 10% of fetal bovine serum (FBS, Invitrogen, Burlington, ON, Canada) and were maintained at 37 °C in a moisture-saturated atmosphere containing 5% CO2.
Ht 29 human colon carcinoma
Manufactured by American Type Culture Collection
Sourced in United States
HT-29 is a human colon carcinoma cell line. It is a widely used model for colorectal cancer research.
Automatically generated - may contain errors
2 protocols using ht 29 human colon carcinoma
1
Cell Culture Protocol for Carcinoma and Melanoma
2
Culturing Common Cancer Cell Lines
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HT-1080 human fibrosarcoma, HT-29 human colon carcinoma and MCF7 human breast carcinoma were purchased from the American Type Culture Collection (Manassas, VA).
M21 human skin melanoma cells were kindly provided by Dr. David Cheresh (University of California, San Diego School of Medicine, USA). Cells were cultured in DMEM medium containing sodium bicarbonate, high glucose concentration, glutamine and sodium pyruvate (Hyclone, Logan, UT) supplemented with 5% of fetal bovine serum (FBS, Invitrogen, Burlington, ON) and were maintained at 37 ºC in a moisture-saturated atmosphere containing 5% CO2.
M21 human skin melanoma cells were kindly provided by Dr. David Cheresh (University of California, San Diego School of Medicine, USA). Cells were cultured in DMEM medium containing sodium bicarbonate, high glucose concentration, glutamine and sodium pyruvate (Hyclone, Logan, UT) supplemented with 5% of fetal bovine serum (FBS, Invitrogen, Burlington, ON) and were maintained at 37 ºC in a moisture-saturated atmosphere containing 5% CO2.
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