Rhabdomyosarcoma Rh30 and Rh18, lung carcinoma A549 and H1299, pancreatic cancer LNCAP and PC3, neuroblastoma SKN-SH and SKN-BE cells were from American Type Culture Collection (ATCC, Rockville, MD). A549, H1299, LNCAP, PC3, SKN-SH and SKN-BE cells were cultured in DMEM (GIBCO) supplemented with 10% heat-inactivated FBS (GIBCO). Rh18 and Rh30 cells were cultured in RMPI 1640 (GIBCO) supplemented with 10% heat-inactivated FBS. 4E-BP1/2 double knock-out MEFs were from Dr. Nahum Sonenberg (McGill University, Canada), and S6K1 knock-out MEFs were provided by Dr. George Thomas (University of Cincinnati). MEF cells were cultured in DMEM supplemented with 10% heat-inactivated FBS. Control and ON-TARGETplusSMARTpool siRNAs of CDK4 were purchased from Dharmacon (Chicago, IL). Lipofectamine 2000 was from Invitrogen (Carlsbad, CA) and transfection of siRNA in cells was performed according to the manufacture's instructions.
A549 and h1299
Manufactured by American Type Culture Collection
A549 and H1299 are human cell lines commonly used in research. A549 is a lung carcinoma cell line, and H1299 is a non-small cell lung cancer cell line. Both are widely used as models for studying various aspects of lung biology and disease.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Lncap, by Thermo Fisher Scientific (1 mentions)
Sk n sh, by American Type Culture Collection (1 mentions)
H1299, by Thermo Fisher Scientific (1 mentions)
Rmpi 1640, by Thermo Fisher Scientific (1 mentions)
Sk n sh, by Thermo Fisher Scientific (1 mentions)
Sk n be, by American Type Culture Collection (1 mentions)
Sk n be, by Thermo Fisher Scientific (1 mentions)
Lncap and pc3, by American Type Culture Collection (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
Beas 2b, by American Type Culture Collection (1 mentions)
2 protocols using a549 and h1299
1
Cell Line Culture and Characterization
2
Lung Cancer Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals,
solvents, reagents, and cell culture media were
purchased from Sigma-Aldrich Co. (St. Louis, MO) and Fisher Scientific
(Pittsburgh, PA) and used without further purification unless otherwise
stated. Nonsmall-cell lung cancer cell lines (A549 and H1299), a human
normal bronchial epithelial cell line (BEAS-2B), and human umbilical
vein endothelial cells (HUVEC) were purchased from American Type Culture
Collection (ATCC, Manassas, VA). The H1299, A549, BEAS-2B, and HUVECs
were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640),
Dulbecco’s modified Eagle medium (DMEM), bronchial epithelial
cell growth medium (BEGM), and endothelial cell medium-2 (EGM TM-2).
All media were supplemented with 10% fetal bovine serum and 1% (w/v)
penicillin–streptomycin. Cell lines were maintained at 37 °C
with a humidified atmosphere of 5% CO2 in an incubator.
Cell lines were regularly monitored for their typical morphology and
contamination under a microscope. These cells were trypsinized and
seeded for in vitro studies.
solvents, reagents, and cell culture media were
purchased from Sigma-Aldrich Co. (St. Louis, MO) and Fisher Scientific
(Pittsburgh, PA) and used without further purification unless otherwise
stated. Nonsmall-cell lung cancer cell lines (A549 and H1299), a human
normal bronchial epithelial cell line (BEAS-2B), and human umbilical
vein endothelial cells (HUVEC) were purchased from American Type Culture
Collection (ATCC, Manassas, VA). The H1299, A549, BEAS-2B, and HUVECs
were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640),
Dulbecco’s modified Eagle medium (DMEM), bronchial epithelial
cell growth medium (BEGM), and endothelial cell medium-2 (EGM TM-2).
All media were supplemented with 10% fetal bovine serum and 1% (w/v)
penicillin–streptomycin. Cell lines were maintained at 37 °C
with a humidified atmosphere of 5% CO2 in an incubator.
Cell lines were regularly monitored for their typical morphology and
contamination under a microscope. These cells were trypsinized and
seeded for in vitro studies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!