We imported the synthesized NRSF and EGFR 3′ UTR gene fragments into the pmir-reporter gene (Beijing Huayueyang Biotechnology). NRSF-mut and EGFR-wt design binding site mutations based on NRSF-mu and EGFR-mu. Epidermal growth factor receptor-WT and MUT were co-transfected into HEK293 T cells with NRSF (Shanghai Beinuo Biotechnology). After 48 hours of transfection, cells were collected and cleaved using a luciferase detection kit (K801-200; Biovision).
Pmir reporter gene
Manufactured by Huayueyang
Sourced in China
The Pmir-reporter gene is a laboratory equipment used for gene expression analysis. It functions as a reporter gene, allowing researchers to monitor and quantify the expression of target genes. The Pmir-reporter gene provides a reliable and efficient tool for studying gene regulation and activity in various biological systems.
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Lab products found in correlation
2 protocols using pmir reporter gene
1
Evaluating NRSF and EGFR Regulatory Interactions
2
Rap2C 3'UTR Luciferase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The synthetic Rap2C 3′UTR gene fragment was imported into pMIR-reporter gene (Huayueyang Biotechnology, Beijing, China). In consonance with the Rap2C-WT binding site, the mutation of Rap2C-MUT binding site was designed. These composite vectors of Rap2C-WT and MUT were co-transfected into HEK293 T cells (Beinuo Biotechnology, Shanghai, China). After 48 hours transfection, the fluorescence intensity was monitored using a correlative luciferase kit (K801-200; Biovision, Mountain View, CA, USA).
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