Jurkat wild-type A3, FADD-deficient Jurkat I 2.1 and RPE-1 hTERT human cell lines were obtained from ATCC (American Type Culture Collection, Rockville, MD, USA). Jurkat wild type, FADD-deficient Jurkat cells were cultured in RPMI containing Glutamax (Invitrogen) supplemented with 15% FCS. RPE-1 hTERT cells were cultured in DMEM-F12 containing Glutamax (Invitrogen) supplemented with 10% FCS. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient centrifugation from blood buffy coats of healthy donors, provided by the Etablissement Français du Sang (EFS). The research protocol was conducted under French legal guidelines. After separation of monocytes by 1 h adhesion step, non-adherent PBMCs (PBL) were harvested. PBL were cultured in RPMI 1640 medium (Gibco, Life technologies, Carlsbad, CA, USA) supplemented with 10% decomplemented fetal calf serum (Life technologies), penicillin (100 IU/ml), and streptomycin (100 µg/ml). All cells were cultured under a 5% CO2 atmosphere at 37 °C.
Rpe 1 htert
Manufactured by American Type Culture Collection
Sourced in United States
RPE-1 hTERT is a continuous human retinal pigment epithelial cell line that has been immortalized through the ectopic expression of the human telomerase reverse transcriptase (hTERT) gene. This cell line maintains the physiological and functional characteristics of normal retinal pigment epithelial cells.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Pen strep, by Wisent (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Fujifilm (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
2 protocols using rpe 1 htert
1
Culturing Jurkat, RPE-1, and PBMC Cells
2
Cell Culture Protocols for Multiple Cell Lines
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RPE1-hTERT, HCT116, and 293T cells were purchased from American Type Culture Collection. HeLa T-Rex Flp-In cells orginated from the laboratory of Stephen Taylor and were a kind gift from Laurence Pelletier. RPE1-hTERT-derived cell lines, 293T-derived cell lines, and HeLa T-Rex Flp-In-derived cell lines were grown in Dulbecco's modified Eagle medium (DMEM) (Gibco/Thermo Fisher, 11965092) supplemented with 10% fetal bovine serum (FBS) (Wisent), 1× GlutaMAX, 1× nonessential amino acids (both Gibco/Thermo Fisher), and 100 U/mL penicillin and 100 µg/mL streptomycin (Pen/Strep) (Wisent). SUM149PT Cas9 cell origin and culture conditions were as described previously (Zimmermann et al. 2018 (link)). HCT116-derived cell lines were grown in McCoy's 5A (Gibco, 1660-082) + 10% FBS (Gibco) + 1% penicilllin–streptomycin mix (Nakalai Tesque, 09367-34) + 1× L-glutamine (final 2 mM; Wako Chemical, 073-05391). RPE1-hTERT Cas9 TP53-KO BRCA1-KO were cultured at 37°C and 3% O2. All other cell lines were grown at 37°C and atmospheric O2. All cell lines were routinely authenticated by STR and tested negative for mycoplasma.
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