We performed the RIP experiments with the Magna RIP RBP immunoprecipitation kit (Millipore) in accordance with the manufacturer’s directions. AsPC-1 and BxPC-3 cells were lysed in complete RIP lysis buffer, and the cell extracts were then mixed with magnetic beads conjugated with specific antibodies or control IgG (Millipore) and incubated for 6 h at 4 °C. To remove the protein, the beads were incubated with proteinase K after washing. Finally, the purified RNA was subjected to qRT-PCR analysis. The EZH2 RIP assay antibody was from Abcam.
Ezh2 rip assay antibody
Manufactured by Abcam
Sourced in United Kingdom
The EZH2 RIP assay antibody is a research-use antibody designed to facilitate the enrichment of RNA-protein complexes containing the EZH2 protein. It can be used in RNA immunoprecipitation (RIP) assays to study the interactions between EZH2 and its associated RNA transcripts.
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2 protocols using ezh2 rip assay antibody
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RIP Assay for EZH2 Binding Partners
2
RIP Assay for EZH2 Binding
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In accordance with the manufacturer’s instructions, we performed RIP experiments using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (EMD Millipore, Billerica, MA, USA). AsPC-1 and BxPC-3 cells were lysed in complete RIP lysis buffer; the cell extracts were mixed with magnetic beads conjugated with specific antibodies or control IgG (EMD Millipore), and incubated for 6 hours at 4°C. To remove the protein, we incubated the extracts with proteinase K after washing the beads. Finally, the purified RNA was subjected to qRT-PCR analysis. The EZH2 RIP assay antibody was purchased from Abcam (Cambridge, UK).
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