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Cathepsin activity fluorometric assay kits

Manufactured by Abcam
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The Cathepsin Activity Fluorometric Assay kits are lab equipment designed to measure the activity of cathepsin enzymes. They use a fluorogenic substrate to detect the proteolytic activity of cathepsins in biological samples.

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Lab products found in correlation

M200 spectrofluorometer, by Tecan (1 mentions)

Close spelling variants of this product

Cathepsin D Activity Fluorometric Assay Kit Cathepsin K Activity Fluorometric Assay Kit Cathepsin L Activity Fluorometric Assay Kit Cathepsin B Activity Fluorometric Assay Kit Fluorometric assay kit Activity assay kits Fluorometric assays

2 protocols using cathepsin activity fluorometric assay kits

1

Cathepsin Activity Fluorometric Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Macrophages were seeded in 6-well plates at a density of 2 × 106 cells per well, and were recovered with a 5 mM EDTA/PBS solution. Cell lysis and measurement of the enzymatic activity was performed using the Cathepsin Activity Fluorometric Assay kits from Biovision specific for each cathepsin and following the manufacturer’s instructions. Essay specificity was verified by treating the cell lysates with specific inhibitors for each cathepsin, provided in the kit. The fluorescence intensity was measured with a spectrofluorometer, Tecan M200.
Pires D., Marques J., Pombo J.P., Carmo N., Bettencourt P., Neyrolles O., Lugo-Villarino G, & Anes E. (2016). Role of Cathepsins in Mycobacterium tuberculosis Survival in Human Macrophages. Scientific Reports, 6, 32247.
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2

Cathepsin Activity Fluorometric Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mø were seeded in six-well plates at a density of 2 × 106 cells per well, and were recovered with a 5 mM EDTA/PBS solution. Cell lysis and measurement of the enzymatic activity was performed using the Cathepsin Activity Fluorometric Assay kits (Biovision) specific for each cathepsin and following the manufacturer’s instructions. Essay specificity was verified by treating the cell lysates with specific inhibitors for each cathepsin, provided in the kit. The fluorescence intensity was measured by the Tecan M200 spectrofluorometer.
Pires D., Bernard E.M., Pombo J.P., Carmo N., Fialho C., Gutierrez M.G., Bettencourt P, & Anes E. (2017). Mycobacterium tuberculosis Modulates miR-106b-5p to Control Cathepsin S Expression Resulting in Higher Pathogen Survival and Poor T-Cell Activation. Frontiers in Immunology, 8, 1819.
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