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Rabbit anti sert

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Rabbit anti-SERT is a primary antibody that specifically binds to the Serotonin Transporter (SERT) protein. It is used as a tool in research applications to detect and study the expression and distribution of SERT in various biological samples.

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Lab products found in correlation

Mouse anti gfap, by Merck Group (1 mentions) Rat anti brdu, by Abcam (1 mentions) Chicken anti gfp, by Aves Labs (1 mentions) Mouse anti actub, by Merck Group (1 mentions) Mouse anti β catenin, by BD (1 mentions) Aqua poly mount mounting medium, by Polysciences (1 mentions) Rabbit anti 5 ht, by Immunostar (1 mentions) Rabbit anti dcx, by Cell Signaling Technology (1 mentions) Rabbit anti synaptophysin, by Abcam (1 mentions) Rabbit anti aciii, by Santa Cruz Biotechnology (1 mentions) Mouse anti mash1, by BD (1 mentions) 4 6 diamidino 2 phenylindol dapi, by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Goat anti doublecortin dcx, by Santa Cruz Biotechnology (1 mentions) Avidin biotin peroxidase complex, by Vector Laboratories (1 mentions) Mouse anti tph, by Santa Cruz Biotechnology (1 mentions) Streptavidin pacific blue, by Thermo Fisher Scientific (1 mentions) Guinea pig anti vglut2, by Merck Group (1 mentions) Neurolucida 360, by MBF Biosciences (1 mentions) Rabbit anti vip, by Immunostar (1 mentions)

4 protocols using rabbit anti sert

1

Immunohistochemical Staining Protocol

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Both wholemounts and sections were incubated for 24–48 hours at 4°C with primary antibodies, followed by 24–48 hours at 4°C with the appropriate secondary a ntibodies (Molecular Probes, Invitrogen). The blocking solutions used were PBS/ 0.5% Triton X-100/ 10% Normal Donkey Serum (NDS) for wholemounts, and PBS / 0.1% Triton X-100/ 5% NDS for sections. For BrdU staining, samples were incubated for 40 min at 37°C w ith 2N HCl followed by 10 min at 25°C with 0.1 M boric acid (pH8.5), prior to incubation with antibodies. For mounting, the wholemounts and sections were embedded with Aqua Poly/Mount mounting medium (Polysciences). Primary antibodies: Mouse anti-AcTub (Sigma, 1:1000), Rabbit anti-5HT (Immunostar, 1:500), Rabbit anti-SERT (Immunostar, 1:500), Rabbit anti-synaptophysin (Abcam, 1:500), Goat anti-vGluT3 (Abcam, 1:200), Rabbit anti-ACIII (Santa Cruz, 1:500), Mouse anti-β-catenin (BD Biosciences, 1:500), Chicken anti-GFP (Aves Labs, 1:500), Rabbit anti-DCX (Cell Signaling, 1:200), Mouse anti-GFAP (Millipore, 1:1000), Mouse anti-Mash1 (BD Biosciences, 1:200) and rat anti-BrdU (Abcam, 1:500).
Tong C.K., Chen J., Cebrián-Silla A., Mirzadeh Z., Obernier K., Guinto C.D., Tecott L.H., García-Verdugo J.M., Kriegstein A, & Alvarez-Buylla A. (2014). Axonal Control of the Adult Neural Stem Cell Niche. Cell stem cell, 14(4), 500-511.
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2

Immunohistological Analysis of Brain Sections

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All immunohistological analyses were conducted using sagittal whole brain sections of four to five male animals per group. Free-floating sections were stained as previously described (Kohl et al. 2012 (link)). The following primary antibodies were used: rat-anti-human a-syn 15G7 (1:20, Enzo Life Science, Lörrach, Germany), rabbit-anti-SERT (1:2000, Immunostar, Hudson, USA), goat-anti-Doublecortin (DCX, 1:1000, Santa Cruz, Dallas, USA), and rabbit-anti-Tph2 (1:1000, provided by K. P. Lesch, Julius Maximilian University of Würzburg, Germany). For chromogenic immunodetection, donkey-anti-rabbit or donkey-anti-goat biotinylated secondary antibody (1:1000, Dianova, Hamburg, Germany) was combined with avidin-biotin-peroxidase complex (1:100, Vector Laboratories, Burlingame, CA, USA). For fluorescent immunodetection, donkey-anti-rabbit antibody conjugated with Alexa Fluor 488 (1:1000, Life Technologies, USA), and donkey-anti-rat antibody coupled with Rhodamine Red™-X (Rhox, 1:500, Dianova, Hamburg, Germany) were used and sections were counterstained with 4′,6′-diamidino-2-phenylindol (DAPI, 1:10.000, Sigma, Hamburg, Germany).
Deusser J., Schmidt S., Ettle B., Plötz S., Huber S., Müller C.P., Masliah E., Winkler J, & Kohl Z. (2015). Serotonergic dysfunction in the A53T alpha-synuclein mouse model of Parkinson’s disease. Journal of neurochemistry, 135(3), 589-597.
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3

Serotonergic System Immunofluorescence Profiling

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Brain tissue preparation, slicing, immunohistochemistry, and immunofluorescence procedures were carried out as described previously [41 (link)]. We performed the following reactions: (1) double staining for cFos and TPH (DR/MR) or cFos and OX (PFA) to identify brain regions and neurochemical systems involved in responses to optical stimulation; (2) single SERT staining (PFA and amygdala-control site) and double TPH/CTB immunofluorescence (DR/MR) to respectively confirm lesioning of local serotonergic fibers and their associated cell bodies; and (3) double SERT/eYFP (PFA) and TPH/eYFP (DR/MR) immunofluorescence to confirm colocalization between the serotonergic system and the viral constructs in the local fibers and cell bodies, respectively.
We used the following primary antibodies: goat anti-CTB (cat. no. 703, List Biological Laboratories; 1:1000–2000), rabbit anti-SERT (cat. no. 24330, ImmunoStar; 1:500–1000), rabbit anti-cFos (cat. no. SC52, Santa Cruz Biotechnology; 1:500), mouse anti-TPH (cat. no. T0678, Sigma-Aldrich; 1:300–1000), and rabbit anti-OX (cat. no. H-003-30, Phoenix Pharmaceuticals; 1:12,000).
Bernabe C.S., Caliman I.F., de Abreu A.R., Molosh A.I., Truitt W.A., Shekhar A, & Johnson P.L. (2024). Identification of a novel perifornical-hypothalamic-area-projecting serotonergic system that inhibits innate panic and conditioned fear responses. Translational Psychiatry, 14, 60.
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4

Multimodal Neuronal Characterization

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Immunohistochemistry on brain slices was performed as previously described (De Marco García et al., 2011 (link)). Electroporated neurons were identified by expression of eGFP (1:1000, Goat Anti-GFP, VRW). Identity of presynaptic partners illuminated by rabies tracing was determined by co-expression of mCherry (1:2000, chicken anti-RFP, VRW). In addition, immunohistochemistry against interneuron markers including reelin (1:500, mouse anti-reelin, VWR) and VIP (1:500, rabbit anti-VIP, Immunostar) was used to determine the identity of starter cells. GCaMP6s expression was evaluated using goat anti-eGFP (1:1000). Guinea pig anti-VGlut2 (1:1000, EMD Millipore) and Rabbit anti-SERT (1:10000, Immunostar) were used in barrel field analysis. Neurons filled with biocytin during whole-cell patch clamp recordings were labeled post hoc with streptavidin (1:1000, Streptavidin Pacific Blue, Thermo Fisher) and anti-reelin (1:1000). Neurons were imaged at 60× with an oil immersion objective (NA 1.4) at 0.5–1 um steps and traced using Neurolucida 360 (2.70.5, 64 bit, MBF Bioscience). Morphological analysis was carried out on NeuroExplorer (Nex Technologies) as previously described (De Marco García et al., 2011 (link); De Marco García and Fishell, 2014 (link)). Images in Figure 1A, Figure 3D and H, Figure 8A, D, and E, Figure S3A and F, and Figure S7A are composite images.
Che A., Babij R., Iannone A.F., Fetcho R.N., Ferrer M., Liston C., Fishell G, & De Marco Garcia N.V. (2018). Layer I interneurons sharpen sensory maps during neonatal development. Neuron, 99(1), 98-116.e7.
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