Overnight cultures of BEAS2B cells, seeded at 4 × 105 cells per well in 6-well plates containing 16-mm coverslips, were infected with RSV at an MOI of 1.0, and 20 h after infection, the cells were fixed with 4% paraformaldehyde, blocked with 3% bovine serum albumin (BSA) in 0.2% Triton X-100, phosphate-buffered saline (PBS) for 10 min, and coimmunostained with polyclonal anti-RSV (1:200; Abcam) and anti-LLT1 (1:200; Novus Biologicals) for 1.5 h, followed by secondary antibodies, anti-goat conjugated to Alexa Fluor 488 (AF488) (RSV) and anti-mouse AF568 (LLT1) (1:1,000; Invitrogen), for 45 min. Images were obtained on a Zeiss 5 Pascal confocal laser scanning microscope using a 63×/1.4 Plan-Apochromat oil lens (averaging 4 times). Images were acquired using Zeiss LSM Image Browser software (4.2.0.121; Zeiss).
Anti rsv
Manufactured by Abcam
Sourced in Spain
Anti-RSV is a lab equipment product designed to detect and analyze the presence of Respiratory Syncytial Virus (RSV) in samples. It functions as a tool for research and diagnostic purposes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anti rsv
1
Immunofluorescence Staining of RSV-Infected BEAS2B Cells
2
Quantification of Viral Protein Levels
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The anti-SH, anti-P, anti-M2 anti-M, anti-NS2 and anti-N have described previously [14 (link), 15 (link), 22 (link)]. The anti-F was obtained from Joes Melero (Madid, Spain), and the anti-G and anti-RSV were purchased from Abcam and Novacastra respectively. The anti-STAT1, anti-pSTAT1, anti-JNK, anti-pJNK, anti-MAPKp38 and anti-pMAPKp38 were purchased from transduction laboratories. The anti-rabbit and anti-mouse IgG conjugated to Alexa488 and Alexa555 (invitrogen) were used in this study. Stock solution of NSC23766 (Calbiochem) was prepared in distilled water (5 mg/ml) and working concentrations (50 μg/ml) were prepared in DMEM + 2%FCS just prior to use. Y27632 (Calbiochem) was prepared in distilled water (5 mg/ml) and working concentrations (10 μg/ml) were prepared in DMEM + 2%FCS just prior to use.
3
Immunofluorescence Imaging of RSV Infection
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3.75 × 105 primary AMs were seeded in a 24-well flat-bottom plate containing glass coverslips. The cells were allowed to adhere for 3 h before exposure to medium, RSV, or UV-RSV. After 21 h the cells were washed with PBS and fixed with 4% paraformaldehyde for 10 min at RT followed by blocking with 3% BSA in 0.2% Triton X/PBS for a further 10 min. The cells were stained with polyclonal anti-RSV (1:200; Abcam) or monoclonal anti-RSV N (1:300; Abcam) and anti-LAMP-1 (1:300; Abcam) antibodies for 1.5 h at RT. This was followed by staining with species-specific secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 568 (1:1,000; Invitrogen) for 45 min in the dark at RT. The coverslips were mounted onto glass slides with ProLong® Gold Antifade Mountant with DAPI (ThermoFisher Scientific). The Zeiss confocal laser-scanning microscope (LSM)-510 on a 100×/1.4 Plan-Apochromat oil lens was used for obtaining images of the cells. Analysis of the images was performed on Fiji, an open-source imaging processing software (ImageJ).
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