Immunohistochemical (IHC) staining experiments were used to detect the expression and abundance of sodium channel NaV1.7 in mouse sciatic nerve tissue. Anti-NaV1.7 antibody [N68/6] (Abcam ab85015) was found to specifically bind to mouse NaV1.7 (0.5 μg/mL). Paraffin-embedded formalin-fixed 5 μm sections were deparaffinized with EZPrep buffer. For IHC detection, a 3,3′-diaminobenzidine (DAB) detection kit (Ventana Medical Systems, Tucson, AZ) was used according to the manufacturer’s instructions. These experiments were performed at the MSKCC Molecular Cytology Core Facility using the Discovery XT processor (Ventana Medical System, Tucson, AZ). Adjacent sections were stained against IgG, to control for non-specific binding to NaV1.7. Sections were counterstained with hematoxylin and eosin (H&E) and coverslipped with Permount (Fisher Scientific, Pittsburgh, PA) for morphological evaluation of tissue characteristics.
N68 6
Manufactured by Abcam
The [N68/6] is a piece of laboratory equipment designed for a specific function. It is a compact and durable device engineered to perform a core task within the research or analytical workflow. The details of its intended use and performance capabilities are not available in this factual and unbiased description.
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2 protocols using n68 6
1
Immunohistochemical Analysis of Nav1.7 Expression in Mouse Sciatic Nerve
2
Immunohistochemical Detection of Nav1.7 in Mouse Sciatic Nerve
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Immunohistochemical (IHC) staining experiments were used to detect the expression and abundance of sodium channel Na V 1.7 in mouse sciatic nerve tissue. Anti-Na V 1.7 antibody [N68/6] (Abcam ab85015) was found to speci cally bind to mouse Na V 1.7 (0.5 µg/mL). Para n-embedded formalin-xed 5 µm sections were depara nized with EZPrep buffer. For IHC detection, a 3,3'-diaminobenzidine (DAB) detection kit (Ventana Medical Systems, Tucson, AZ) was used according to the manufacturer's instructions. These experiments were performed at the MSKCC Molecular Cytology Core Facility using the Discovery XT processor (Ventana Medical System, Tucson, AZ). Adjacent sections were stained against IgG, to control for non-speci c binding to Na V 1.7. Sections were counterstained with hematoxylin and eosin (H&E) and coverslipped with Permount (Fisher Scienti c, Pittsburgh, PA) for morphological evaluation of tissue characteristics.
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