Ab151376

Manufactured by Abcam

Sourced in United Kingdom, China

Ab151376 is a laboratory equipment product. It is designed to perform a specific function in the laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Alzet osmotic minipump, by Durect (1 mentions) C57bl 6j mice, by Abcam (1 mentions) Tlr4 ko mice, by Jackson ImmunoResearch (1 mentions) Clodronate liposomes cls, by Liposoma (1 mentions)

2 protocols using ab151376

CTRP1 Modulates Cardiac Hypertrophy in Mice

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In this experimental study, C57BL6J male mice were
purchased from Beijing Huafukang Biological Co.
Ltd. (Beijing, China). The mice were divided into four
groups: vehicle-NS (normal saline), CTRP1-NS, vehicleisoproterenol (ISO), and CTRP1-ISO. Mice from the two
ISO groups were injected subcutaneously with ISO (10
mg/kg/day for 3 days and then 5 mg/kg/day for 11 days).
The mice in the two CTRP1 groups (n=12 for each group),
following the last injection of ISO or NS, received 200
μg/kg recombinant CTRP1 full-length protein (ab151376,
Abcam, Cambridge, UK) or vehicle solution for 2 weeks
using an Alzet osmotic minipump (Durect, Cupertino,
CA, USA). Animal experiments were performed per the
guidelines of the National Institutes of Health Guidelines
for the Care and Use of Laboratory Animals (NIH
Publication No. 85-23, revised in 1996). All the animal
experiments were reviewed and approved by Xiangyang
Central Hospital's Animal Care and Use Committee.

Li C., Ying S., Wu X., Zhu T., Zhou Q., Zhang Y., Liu Y., Zhu R, & Hu H. (2022). CTRP1 Aggravates Cardiac Fibrosis by Regulating The NOX2/P38 Pathway in Macrophages. Cell Journal (Yakhteh), 24(12), 732-740.

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CTRP1 Modulation in Post-MI Mice

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CTRP1-KO mice were purchased from Cyagen Biosciences (Guangzhou, China, KOCMP-07979). C57BL6J mice (male) were purchased from Beijing Huafukang Biology Co., Ltd (Beijing, China). TLR4-KO mice were purchased from Jackson Laboratory (stock no: 029015). C57BL6J mice purchased from the Chinese Academy of Medical Sciences (Beijing, China) were subjected to intraperitoneal injection of recombinant human CTRP1 full-length protein (Abcam, ab151376; 200 μg/kg) every other day from days 7 to 28 post MI). For the macrophage clearance experiment, mice were subjected to intravenous injection of clodronate liposomes (CLs; 150 mL; 5 mg/mL; Liposoma, The Netherlands) at 10- and 14-days post MI. For the TLR4 deficiency experiment, TLR4-KO mice were subjected to intraperitoneal injection of recombinant human CTRP1 (200 μg/kg, from 7 days to 28 days post MI). All animal experiments were approved by the Institutional Animal Care and Use Committee of Huai’an First People’s Hospital, Nanjing Medical University (Huai’an, China).

Gu Y., Hu X., Ge P.B., Chen Y., Wu S, & Zhang X.W. (2021). CTRP1 Aggravates Cardiac Dysfunction Post Myocardial Infarction by Modulating TLR4 in Macrophages. Frontiers in Immunology, 12, 635267.

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