α cd69

Manufactured by BioLegend

α-CD69 is a monoclonal antibody that binds to the CD69 antigen, which is a type II C-type lectin-like receptor expressed on the surface of activated T cells, B cells, and natural killer cells. CD69 is an early activation marker that is upregulated upon cellular activation.

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Lab products found in correlation

α cd45, by BioLegend (3 mentions) Facs lysis buffer, by BD (2 mentions) α cd4, by BioLegend (2 mentions) α cd40, by BioLegend (2 mentions) Collagenase type 4, by Thermo Fisher Scientific (2 mentions) α cd44, by BD (2 mentions) α cd103, by BioLegend (2 mentions) α ifnar1, by BioLegend (2 mentions) Gentlemacs, by Merck Group (2 mentions) α cd8α, by BioLegend (2 mentions) Dnase 1, by Merck Group (2 mentions) α cd8, by BioLegend (1 mentions) True nuclear transcription factor buffer set, by BioLegend (1 mentions) α cd25, by BioLegend (1 mentions) α ki67, by BioLegend (1 mentions) α cd107a, by BioLegend (1 mentions) αifnγ, by BioLegend (1 mentions) Ultra leaf purified anti mouse cd16 32, by BioLegend (1 mentions) Cytoflex lx, by FlowJo (1 mentions) Brilliant stain buffer, by BD (1 mentions)

3 protocols using α cd69

Comprehensive Immune Cell Profiling of Mouse Tissues

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At the designated times after infection, mice were euthanized, and tissues were perfused with PBS by cardiac injection. Lungs and/or dLN were harvested and single cell suspensions were created by enzymatic digestion with type IV collagenase (Fisher/Worthington) and DNase I (Sigma) and GentleMacs (Miltemyi) processing.
For flow cytometry staining, single cell homogenates were blocked in 2% rat serum for 10 min at room temperature, incubated with specified antibodies for 30 min on ice, fixed with BD FACS Lysis buffer for 10 min at room temperature, resuspended in PBS, and run on the LSRII (BD). Samples were analyzed using FlowJo software (BD).
The following anti-mouse antibodies conjugated to fluorochromes were utilized for flow cytometry: (company, clone) α-CD3ε (eBioscience/Invitrogen, 145–2C11), α-CD4 (Biolegend, GK1.5), α-CD8α (Biolegend, 53–6.7), α-CD11b (eBioscience/Invitrogen, M1/70), α-CD11c (eBioscience/Invitrogen, N418), α-CD19 (eBioscience/Invitrogen, 1D3), α-CD40 (Biolegend, HM40–3), α-CD44 (BD, IM7), α-CD45.1 (eBioscience/Invitrogen, A20), α-CD45.2 (Biolegend, 104), α-CD69 (Biolegend, H1.2F3), α-CD103 (Biolegend, 2E7), α-F4/80 (eBioscience/Invitrogen, BM8), α-IFNAR1 (Biolegend, MAR1–5A3), α-IFNγ (eBioscience/Invitrogen, XMG1.2), α-MHCII (eBioscience/Invitrogen, M5/114.15.2), α-TNFα (eBioscience/Invitrogen, MP6-XT22).

Hemann E.A., Green R., Turnbull J.B., Langlois R.A., Savan R, & Gale M J.r. (2019). Interferon-λ modulates dendritic cells to facilitate T cell immunity during infection with influenza A virus. Nature immunology, 20(8), 1035-1045.

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Comprehensive Tumor Immune Profiling

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Chopped tumor tissues were enzymatically digested using the dissociation buffer supplemented with 1 mg/ml Collagenase B (11088807001, Roche) and 1 mg/ml Hyaluronidase (H3506, Sigma-Aldrich) for 1 h at 37 °C. The prepared single-cell suspensions were filtered through 40-μm nylon meshes (352340, Corning) and centrifuged at 400 g for 5 min. Then, the centrifuged cells were treated with red blood cell lysis buffer (C3702, Beyotime). Subsequently, the cells were dyed with Fixable Viability Stain 780 (565388, BD), and Fc receptors were blocked with Ultra-LEAF™ Purified anti-mouse CD16/32 (101320, BioLegend). Cells were then fluorescently stained with the following detection antibodies: α-CD45 (103132, BioLegend), α-CD3 (100206, BioLegend), α-CD8 (100706, BioLegend), α-Ki67 (151215, BioLegend), α-CD69 (104536, BioLegend), α-CD25 (102012, BioLegend), α-CD107a (121629, BioLegend), α-Granzyme-B (372214, BioLegend), α-IFN-γ (505838, BioLegend). Brilliant Stain Buffer (563794, BD Biosciences) and True-Nuclear™ Transcription Factor Buffer Set (424401, BioLegend) were used in this assay. Flow cytometry was performed using Beckman CytoFLEX LX, and the data were analyzed by FlowJo_V10.

Niu M., Yi M., Wu Y., Lyu L., He Q., Yang R., Zeng L., Shi J., Zhang J., Zhou P., Zhang T., Mei Q., Chu Q, & Wu K. (2023). Synergistic efficacy of simultaneous anti-TGF-β/VEGF bispecific antibody and PD-1 blockade in cancer therapy. Journal of Hematology & Oncology, 16, 94.

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Comprehensive Immune Cell Profiling of Mouse Tissues

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