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The UAS-mGFP is a genetic construct used for fluorescent labeling of cells and tissues in Drosophila. It contains the coding sequence for a membrane-tethered form of the green fluorescent protein (mGFP) under the control of the Upstream Activation Sequence (UAS) promoter. This construct can be expressed in a cell-specific manner when combined with a Gal4 driver line, allowing for targeted visualization of the labeled cells.

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Lab products found in correlation

Df 2l bsc278, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 rfp, by Bloomington Drosophila Stock Center (1 mentions) Uas bsk, by Bloomington Drosophila Stock Center (1 mentions) Ser gal4, by Bloomington Drosophila Stock Center (1 mentions) Vglut gal4, by Bloomington Drosophila Stock Center (1 mentions) Tdc2 gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas reaper, by Bloomington Drosophila Stock Center (1 mentions) Uas tetxlc, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using uas mgfp

1

Drosophila Genetics and Circadian Rhythms

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Drosophila strains were raised and maintained on standard agar food at 22° to 25° C in light:dark conditions. Behavior and immunofluorescence experiments were performed using adult males, while most FACS sorting experiments used an equal mix of male and female adults. pdfGAL4 [45 (link)], Clk4.1GAL4 [34 (link), 35 (link)], elavGAL4 [97 (link)], timGAL4-62 [98 (link)], pdfGAL80 [32 (link)], per01 [99 (link)], perS [99 (link)], perL [99 (link)], tim01 [100 (link)], timS1 [60 (link)], timUL [61 (link)], cryb [11 (link)], cry01 [83 (link)], ClkJrk [101 (link)], and UAS-PRL-1 [56 ] have been described previously. Df(2L) BSC278, UAS-mGFP, and w1118 iso31 were obtained from Bloomington Drosophila stock center (BDSC; Bloomington, IN). PRL1{LL07771-Pbac}and pGAWB{NP2526} were obtained from the Drosophila genetics resource center (DGRC; Kyoto). PRL-1 RNAi and UAS-dcr2 strains were obtained from the Vienna Drosophila Resource Center (VDRC; Vienna).
Kula-Eversole E., Lee D.H., Samba I., Yildirim E., Levine D.C., Hong H.K., Lear B.C., Bass J., Rosbash M, & Allada R. (2020). Phosphatase of Regenerating Liver-1 selectively times circadian behavior in darkness via function in PDF neurons and dephosphorylation of TIMELESS. Current biology : CB, 31(1), 138-149.e5.
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2

Drosophila Genetic Manipulation Protocol

Cited 2 times
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Flies were kept on a cornmeal and agar medium at 25 °C according to standard protocols. Drosophila strains used include: sna1 (25127), sna18 (3299), UAS-sna-IRB (28679), UAS-mGFP (32197), bsk1 (3088), UAS-Bsk, UAS-dFoxO (9575), Ser-GAL4 (6791), Sco/CyO (2555), UAS-mCD8-RFP (32219), UAS-dsh-IR (31306), wor1(3155), wor4 (25170) and the deficiency kit were obtained from Bloomington Drosophila stock center. UAS-sna-IRV (6263), UAS-puc-IR (3018), UAS-dlg-IR (41136), and UAS-wg-IRV (13352) were obtained from Vienna Drosophila RNAi center. UAS-wg-IRN (4889R-4), UAS-esg-IRN-1 (3758R-1), and UAS-esg-IRN-2 (3758R-5) were received from Fly Stocks of National Institute of Genetics (NIG-FLY). UAS-GFP-IR (0355) and UAS-wor-IR (GL00186) were obtained from TsingHua Fly Center. GMR-GAL453 (link); Sd-GAL4 and ptc-GAL439 (link); UAS-Egr, UAS-EgrW, and UAS-Hid14 (link); UAS-BskDN, UAS-Puc, and pucE6942 (link); sev-GAL4, UAS-dTAK1, UAS-HepCA, UAS-HepWT, UAS-LacZ, pnr-GAL4, and UAS-GFP22 (link),29 (link),54 (link); dFoxOΔ94 and UAS-dFoxO-IR39 (link) were previously described. UAS-Sna74b fly was a kind gift of J. Kumar. For all fly cross experiments, healthy unmated male and female parents were randomly assigned to different groups. Double-blinded method was employed during the experiments.
Wu C., Li Z., Ding X., Guo X., Sun Y., Wang X., Hu Y., Li T., La X., Li J., Li J.A., Li W, & Xue L. (2019). Snail modulates JNK-mediated cell death in Drosophila. Cell Death & Disease, 10(12), 893.
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Drosophila Neuronal Lineage Tracing

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The following strains were used: Janelia Gal4 lines 24G08 Gal4 (Bloomington stock 49316) and 31B08 Gal4 (Bloomington stock 49351), Tdc2 Gal4 (Bloomington stock 9313), VGlut Gal4 (Bloomington stock 26160), VGlut MiMIC RMCE line with EGFP reporter (and GFDTF tagged) (Nagarkar-Jaiswal et al., 2015 (link)) (Bloomington stock 59411), UAS mGFP (Bloomington stocks 35839), UAS reaper (Bloomington stock 5824), UAS TeTxLC (Bloomington stock 28997). All crosses were raised at 25°C. The white Canton S (wCS) strain (outcrossed to CS, from Jean-Maurice Dura, IGH Montpellier, France) was used as wild type control and was crossed with the different Gal4 lines for control experiments.
Pons M., Soulard C., Soustelle L., Parmentier M.L., Grau Y, & Layalle S. (2017). A New Behavioral Test and Associated Genetic Tools Highlight the Function of Ventral Abdominal Muscles in Adult Drosophila. Frontiers in Cellular Neuroscience, 11, 371.
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